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Establishment and Maintenance of Gnotobiotic American Cockroaches (Periplaneta americana)

Article DOI: 10.3791/61316-v • 06:09 min • May 28th, 2021
May 28th, 2021 •
1, 1, 1
1Department of Microbiology, University of Georgia

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This protocol is used in establishing and maintaining gnotobiotic American cockroaches (Periplaneta americana) by surface sterilizing the egg cases (oothecae) prior to hatching. These gnotobiotic insects contain their vertically transmitted Blattabacterium endosymbionts but have axenic guts.

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Gnotobiotic American Cockroaches Periplaneta Americana Gut Microbial Community Host Health Microbiome Assembly Gnotobiotic Protocol Bench-top Gnotobiotic Facilities Gravid Females Oothecae Forceps Cardboard Tubes Stock Tank Plastic Container Petroleum Jelly Maternity Ward Sodium Dodecyl Sulfate
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