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Neisseria meningitidis Infection of Induced Pluripotent Stem-Cell Derived Brain Endothelial Cells

Article DOI: 10.3791/61400-v • 10:12 min • July 14th, 2020
July 14th, 2020 •
1, 2, 1, 1,2
1Institute for Hygiene and Microbiology, University of Würzburg, 2Department of Biological Sciences, University of Alabama

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The protocol described here highlights the major steps in the differentiating induced pluripotent stem-cell derived brain-like endothelial cells, the preparation of Neisseria meningitidis for infection, and sample collection for other molecular analyses.

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Neisseria Meningitidis Meningococcal Meningitis Brain Endothelial Cells Host-pathogen Interactions In Vivo Model Systems Human-based Model Tight Junctions Trans-endothelial Electrical Resistance (TEER) In Vitro Models Primary BECs Immortalized BECs Induced Pluripotent Stem-cells (iPSCs) IPSC-derived BECs (iPSC-BECs) Barrier Destruction Innate Immune Activation Bacterial Interaction
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