Waiting
Processando Login

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biochemistry / Extraction and Quantification of Soluble,…
JoVE Journal
Biochemistry

This Conteúdo is Open Access.

JoVE Journal Biochemistry
Extraction and Quantification of Soluble, Radiolabeled Inositol Polyphosphates from Different Plant Species using SAX-HPLC
Construction of a Refrigerator System for Leachate Collection to Study Mineral Weathering (Video) | JoVE
Journal (Biochemistry)
Construction of a Refrigerator System for Leachate Collection to Study Mineral Weathering (Video) | JoVE
Production of Recombinant PRMT Proteins using the Baculovirus Expression Vector System
Journal (Biochemistry)
Production of Recombinant PRMT Proteins using the Baculovirus Expression Vector System
 

Extraction and Quantification of Soluble, Radiolabeled Inositol Polyphosphates from Different Plant Species using SAX-HPLC

Article DOI: 10.3791/61495-v • 09:01 min • June 26th, 2020
June 26th, 2020 •
1, 1, 1, 1
1Department of Plant Nutrition, Institute of Crop Science and Resource Conservation, University of Bonn

Capítulos

Resumo
Automatic Translation

Here we describe strong anion exchange high-performance liquid chromatography of [3H]-myo-inositol-labeled seedlings which is a highly sensitive method to detect and quantify inositol polyphosphates in plants.

Tags

Extraction Quantification Soluble Radiolabeled Inositol Polyphosphates Plant Species SAX-HPLC Biosynthesis Plant Metabolism Development Radioactively Labeled Precursor Detection Inositol Phosphate Species HPLC Analysis Setup Critical Steps System Setup HPLC Pumps Buffer A Buffer B Piston Seal Wash Dynamic Mixer Injection Valve Sample Loop Capacity Capillaries Column Fraction Collector Lotus Seeds Petri Dishes Solid Growth Media
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter