A Chronic Sleep Fragmentation Model using Vibrating Orbital Rotor to Induce Cognitive Deficit and Anxiety-Like Behavior in Young Wild-Type Mice

Yi Xie; Sai-Yue Deng; Si-Miao Chen; Xue-Jiao Chen; Wen-Wen Lai; Li-Fang Huang; Li Ba; Wei Wang; Feng-Fei Ding

doi:10.3791/61531

Um modelo de fragmentação crônica do sono usando rotor orbital vibrante para induzir déficit cogn...

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JoVE Journal Behavior

A Chronic Sleep Fragmentation Model using Vibrating Orbital Rotor to Induce Cognitive Deficit and Anxiety-Like Behavior in Young Wild-Type Mice

Um modelo de fragmentação crônica do sono usando rotor orbital vibrante para induzir déficit cognitivo e comportamento semelhante à ansiedade em camundongos jovens do tipo selvagem

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06:23 min

September 22, 2020

DOI: 10.3791/61531-v

Yi Xie¹, Sai-Yue Deng¹, Si-Miao Chen¹, Xue-Jiao Chen¹, Wen-Wen Lai¹, Li-Fang Huang¹, Li Ba¹, Wei Wang^1,2, Feng-Fei Ding^1,3

¹Department of Neurology, Tongji Hospital, Tongji Medical College,Huazhong University of Science and Technology, Wuhan 430000, China, ²Key Laboratory of Neurological Diseases of Chinese Ministry of Education, The School of Basic Medicine, Tongji Medical College,Huazhong University of Science and Technology, Wuhan 430000,China, ³Department of Pharmacology, School of Basic Medical Science; State Key Laboratory of Medical Neurobiology,Institutes of Brain Science and Collaborative Innovation Center for Brain Science, Fudan University, Shanghai 200032, China

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Overview

This article presents a protocol for a chronic sleep fragmentation (CSF) model using an electrically controlled orbital rotor. This model induces cognitive deficits and anxiety-like behavior in young wild-type mice, allowing for the exploration of chronic sleep disturbance and related disorders.

Key Study Components

Area of Science

Neuroscience
Behavioral studies
Sleep research

Background

Chronic sleep disturbances are linked to neurodegenerative diseases.
The study aims to provide a stable protocol for modeling sleep interruptions.
This model minimizes handling and standardizes interventions among experimental groups.
It can be applied to various disease models, including depression and Alzheimer's disease.

Purpose of Study

To model frequent interruptions during sleep in mice.
To investigate the impacts of chronic sleep disturbance on behavior and pathology.
To facilitate repeatable outcomes across different experiments.

Methods Used

Random assignment of 20 to 28 gram, 8 to 10-week-old wild-type male mice.
Use of CSF modeling and control cages to prevent social isolation stress.
Application of an electrically controlled orbital rotor for sleep fragmentation.
Behavioral assessments to evaluate cognitive deficits and anxiety-like behavior.

Main Results

Confirmed cognitive deficits in mice subjected to chronic sleep fragmentation.
Induction of anxiety-like behavior in the experimental group.
Standardized outcomes across different experimental setups.
Potential applications in studying chronic sleep disturbances in various disease contexts.

Conclusions

The CSF model is effective for studying the effects of chronic sleep disturbance.
This protocol can aid in understanding the pathogenesis of related disorders.
Future research can leverage this model for various neurodegenerative diseases.

Frequently Asked Questions

What is the chronic sleep fragmentation model?

It is a protocol that induces sleep disturbances in mice to study cognitive and behavioral effects.

How does the model affect mice?

It leads to cognitive deficits and anxiety-like behavior, mimicking human sleep disturbances.

What are the potential applications of this model?

It can be used to study various diseases, including depression and Alzheimer's disease.

What age and weight of mice are used in the study?

The study uses 8 to 10-week-old male wild-type mice weighing 20 to 28 grams.

How are the mice assigned to experimental groups?

Mice are randomly assigned to CSF modeling and control cages to avoid social isolation stress.

What behavioral assessments are conducted?

Behavioral assessments evaluate cognitive deficits and anxiety-like behavior in the mice.

Apresentado aqui é um protocolo para fragmentação crônica do sono (CSF) modelo alcançado por um rotor orbital eletricamente controlado, que poderia induzir déficit cognitivo confirmado e comportamento semelhante à ansiedade em camundongos jovens do tipo selvagem. Este modelo pode ser aplicado para explorar a patogênese da perturbação crônica do sono e distúrbios relacionados.

O distúrbio crônico do sono leva à patogênese da doença neurodegenerativa. Gostaríamos de fornecer um protocolo simples, estável e de longo prazo para modelar interrupções frequentes durante o sono humano para abordar essa questão. Esses ratos minimizam o manuseio de trabalhos e os compradores artificiais.

Ele pode padronizar intervenções entre camundongos dentro do mesmo grupo experimental e entre diferentes experimentos para produzir resultados comportamentais e patológicos repetíveis. Este modelo pode ser aplicado a uma variedade de modelos de doenças, como depressão e doença de Alzheimer, para estudar os impactos do distúrbio crônico do sono na presença de uma doença subjacente. Comece atribuindo aleatoriamente de três a cinco camundongos machos adultos de 20 a 28 gramas, de 8 a 10 semanas de idade, para modelar o LCR e controlar gaiolas para evitar o estresse do isolamento social.

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