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Journal / Developmental Biology / Drosophila Embryo Preparation and Microinjection…
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Developmental Biology

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JoVE Journal Developmental Biology
Drosophila Embryo Preparation and Microinjection for Live Cell Microscopy Performed using an Automated High Content Analyzer
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Preparation of Drosophila Larval and Pupal Testes for Analysis of Cell Division in Live, Intact Tissue
Journal (Developmental Biology)
Preparation of Drosophila Larval and Pupal Testes for Analysis of Cell Division in Live, Intact Tissue
 

Drosophila Embryo Preparation and Microinjection for Live Cell Microscopy Performed using an Automated High Content Analyzer

Article DOI: 10.3791/61589-v • 05:52 min • January 19th, 2021
January 19th, 2021 •
1,2, 2, 1
1Department of Biology, San Francisco State University, 2Department of Biochemistry & Biophysics, UCSF Mission Bay

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Presented here is a protocol to microinject and simultaneously image multiple Drosophila embryos during embryonic development using a plate-based, high content imager.

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Drosophila Embryo Preparation Microinjection Live Cell Microscopy Automated High Content Analyzer Sample Preparation Multi-point Acquisition Inverted Microscope Embryo Alignment Ventral Side Orientation Heptane Glue Coverslip Spacer Razor Blade Stainless Steel Spatula 3.5 Centimeter Dish Stereo Microscope Halocarbon Oil
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