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Demonstration av heterologa komplex som bildas av Golgi-Resident Typ III Membranproteiner med split luciferas komplementeringsanalys
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Demonstration av heterologa komplex som bildas av Golgi-Resident Typ III Membranproteiner med split luciferas komplementeringsanalys

Article DOI: 10.3791/61669-v • 05:28 min • September 10th, 2020
September 10th, 2020 •
1, 1, 1
1Laboratory of Biochemistry, Faculty of Biotechnology, University of Wroclaw

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Protokollet som presenteras här är avsett att visa förekomsten av heterologa interaktioner mellan Golgi-resident typ III membran proteiner med cytoplasmiskt exponerade N- och/eller C-termini i levande däggdjursceller med den senaste varianten av den delade luciferas komplementering assay.

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Biologi nummer 163 split luciferas komplementeringsanalys protein-proteininteraktioner protonpumpshämmare luminiscens bioluminescens heterologa komplex Golgi-apparatur typ III-membranproteiner nukleotidsockertransportörer NSTs transient transfection
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