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Journal / Genetics / In Vivo CRISPR/Cas9 Screening for samtidig å…
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JoVE Journal Genetics
In Vivo CRISPR/Cas9 Screening for samtidig å evaluere genfunksjon i musehud og munnhule
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In Vivo CRISPR/Cas9 Screening for samtidig å evaluere genfunksjon i musehud og munnhule

Article DOI: 10.3791/61693-v • 07:52 min • November 2nd, 2020
November 2nd, 2020 •
1, 1,2, 1,2, 3, 1,2
1Centre for Molecular and Systems Biology, Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, 2Department of Molecular Genetics, University of Toronto, 3Department of Cell and Developmental Biology, Sackler Faculty of Medicine, Tel Aviv University

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Her beskriver vi en rask og direkte in vivo CRISPR/Cas9 screeningmetodikk ved hjelp av ultralydstyrte i utero embryonale lentivirale injeksjoner for samtidig å vurdere funksjoner av flere gener i huden og munnhulen til immunokokkmus.

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Denne måneden i JoVE utgave 165 in vivo CRISPR ultralydstyrt mikroinjeksjon langhale CRISPR-bibliotek musemodeller av kreft rask in vivo-skjerm
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