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Isolation, Proliferation and Differentiation of Rhesus Macaque Adipose-Derived Stem Cells

Article DOI: 10.3791/61732-v • 07:33 min • May 26th, 2021
May 26th, 2021 •
1,2, 1,2, 1,2
1Department of Physiology, Louisiana State University Health Sciences Center - New Orleans, 2Comprehensive Alcohol-HIV/AIDS Research Center, Louisiana State University Health Sciences Center - New Orleans

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In this article, we describe isolation of rhesus macaque derived adipose-derived stem cells (ADSCs) using an enzymatic tissue digestion protocol. Next, we describe ADSC proliferation which includes cell detachment, counting and plating. Lastly, ADSC differentiation is described using specific adipogenic inducing agents. Additionally, we describe staining techniques to confirm differentiation.

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Isolation Proliferation Differentiation Rhesus Macaque Adipose-derived Stem Cells Metabolic Assays Insulin Stimulated Glucose Uptake Lipogenesis Stimulated Lipolysis Sterile Working Environment Disinfecting Biosafety Hood PBS Buffer Cell Culture Dishes Adipose Sample Wash Minced Tissue Collagenase Buffer Neutralize Enzyme Activity ADSC Growth Medium
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