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JoVE Journal Bioengineering
Single-Cell Optical Action Potential Measurement in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes
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Single-Cell Optical Action Potential Measurement in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes

Article DOI: 10.3791/61890-v • 08:39 min • December 22nd, 2020
December 22nd, 2020 •
1,2, 3, 1,2,4
1Institute of Pharmacology and Toxicology, University Medical Center Goettingen, Goettingen, Germany, 2DZHK (German Center for Cardiovascular Research), Partner Site Goettingen, Germany, 3Cairn Research Ltd, Faversham, United Kingdom, 4Cluster of Excellence "Multiscale Bioimaging: from Molecular Machines to Networks of Excitable Cells" (MBExC), University of Goettingen, Germany

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Here we describe optical acquisition and characterization of action potentials from induced pluripotent stem cell derived cardiomyocytes using a high-speed modular photometry system.

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Single-Cell Optical Action Potential Measurement Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes High Throughput Measurements Cellular Electrophysiology Induced Pluripotent Stem Cells Patch Clamp Technique Large-scale Toxicity Screening Programs In Vitro Proarrhythmia Assay Coating Cover Slips Basement Membrane Matrix Plating Medium Voltage Sensitive Dye Imaging Epifluorescence Microscope Magnification Lens LED Illumination
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