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DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

Article DOI: 10.3791/62073-v • 09:26 min • December 29th, 2021
December 29th, 2021 •
1, 1, 1
1Institute of Biomedical Engineering, University of Toronto

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We describe the engineering of a novel DNA-tethered T7 RNA polymerase to regulate in vitro transcription reactions. We discuss the steps for protein synthesis and characterization, validate proof-of-concept transcriptional regulation, and discuss its applications in molecular computing, diagnostics, and molecular information processing.

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DNA-Tethered RNA Polymerase Programmable In Vitro Transcription Molecular Computation Artificial Nucleic Acid Transcription Factors Gene Regulatory Architecture DNA-based Circuits Transcriptional Circuits Single-stranded BG Oligonucleotide Dilutions SNAP Buffer SNAP T7 RNAP RNAP Control DNA Control Protein Loading Dye Gel Electrophoresis Nucleic Acid Staining
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