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JoVE Journal Developmental Biology
Etablera ett epidermalt sfäroidkultursystem med hög genomströmning för att modellera Keratinocyte stamcellsplastitet
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Etablera ett epidermalt sfäroidkultursystem med hög genomströmning för att modellera Keratinocyte stamcellsplastitet

Article DOI: 10.3791/62182-v • 10:03 min • January 30th, 2021
January 30th, 2021 •
1,2, 3, 4, 5, 6, 1
1Department of Pathology, Microbiology and Immunology, University of South Carolina School of Medicine, 2Department of Dermatology, Brigham and Women's Hospital, Harvard Medical School, 3Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine Baltimore, 4Department of Pathology, Albert Einstein College of Medicine, 5Department of Pediatrics, Vanderbilt University Medical Center, 6Department of Drug Discovery and Biomedical Sciences, College of Pharmacy, University of South Carolina

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Här beskriver vi ett protokoll för systematisk odling av epidermala sfäroider i 3D suspension kultur. Detta protokoll har omfattande tillämpningar för användning i en mängd olika epitelvävnadstyper och för modellering av flera mänskliga sjukdomar och tillstånd.

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Utvecklingsbiologi Utgåva 167 Anoikis cellplastik epitel epidermala stamceller epidermosfär mänskliga keratinocyter sfäroider 3D-kultur sårläkning
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