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JoVE Journal Developmental Biology
Generering og udvidelse af humane kardiomyocytter fra patientens perifere blod mononukleare celler
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Generering og udvidelse af humane kardiomyocytter fra patientens perifere blod mononukleare celler

Article DOI: 10.3791/62206-v • 05:38 min • February 12th, 2021
February 12th, 2021 •
1,2, 3, 1,2, 1,2,4, 1,2,5, 3, 1,2,5,6
1Center for Cardiovascular Research, The Abigail Wexner Research Institute, Nationwide Children’s Hospital, 2The Heart Center, Nationwide Children’s Hospital, 3Department of Physiology and Cell Biology, The Ohio State University College of Medicine, 4Department of Anatomy, The Ohio State University College of Medicine, 5MCDB Graduate Program, The Ohio State University, 6Department of Pediatrics, The Ohio State University College of Medicine

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Her præsenterer vi en protokol til robust at generere og udvide menneskelige kardiomyocytter fra patientens perifere blodmonnukleare celler.

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Udviklingsbiologi Udgave 168 perifere blodmonnukleare celler PBBC'er menneskeskabte pluripotente stamceller iPSCs hjertedifferentiering iPSC omprogrammering kardiomyocyt ekspansion.
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