Waiting
Processando Login

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / Levering af Cas9/sgRNA…
JoVE Journal
Biology

This Conteúdo is Open Access.

JoVE Journal Biology
Levering af Cas9/sgRNA Ribonukleoproteinkomplekset i udødeliggjorte og primære celler via viruslignende partikler ("nanoblade")
Immunophenotyping and Cell Sorting of Human MKs from Human Primary Sources or Differentiated In Vitro from Hematopoietic Progenitors
Journal (Biology)
Immunophenotyping and Cell Sorting of Human MKs from Human Primary Sources or Differentiated In Vitro from Hematopoietic Progenitors
Analysis of Protein Import into Chloroplasts Isolated from Stressed Plants
Journal (Biology)
Analysis of Protein Import into Chloroplasts Isolated from Stressed Plants
 
Click here for the English version

Levering af Cas9/sgRNA Ribonukleoproteinkomplekset i udødeliggjorte og primære celler via viruslignende partikler ("nanoblade")

Article DOI: 10.3791/62245-v • 09:23 min • March 31st, 2021
March 31st, 2021 •
2, 1, 1, 1
1LBMC, Laboratoire de Biologie et Modélisation de la Cellule Univ Lyon, ENS de Lyon, Université Claude Bernard Lyon 1, CNRS, UMR 5239, INSERM, U1210, Lyon, 69007, France, 2CIRI, Centre International de Recherche en Infectiologie Univ Lyon, Inserm, U1111, Université Claude Bernard Lyon 1, CNRS, UMR5308, ENS de Lyon, F-69007, Lyon, France

Capítulos

Resumo
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Vi har udviklet en enkel og billig protokol til at indlæse Cas9 / single-guide RNA (sgRNA) ribonukleoproteinkomplekser i viruslignende partikler. Disse partikler, kaldet "Nanoblades", muliggør effektiv levering af Cas9 / sgRNA-komplekset i udødeliggjorte og primære celler såvel som in vivo.

Tags

Biologi Udgave 169 CRISPR Cas9 ribonukleoprotein RNP Murine Leukæmi Virus Viruslignende partikler VLP viral vektor proteinlevering
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter