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Detectie van glycosaminoglycanen door polyacrylamide gel-elektroforese en zilverkleuring
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Detectie van glycosaminoglycanen door polyacrylamide gel-elektroforese en zilverkleuring

Article DOI: 10.3791/62319-v • 05:57 min • February 25th, 2021
February 25th, 2021 •
1,2, 3,4, 1, 1, 3, 1,5
1Department of Medicine, University of Colorado Anschutz Medical Campus, 2Medical Scientist Training Program, University of Colorado Anschutz Medical Campus, 3Department of Chemistry and Chemical Biology, Rensselaer Polytechnic Institute, 4Department of Health Sciences, Curtin University, 5Department of Medicine, Denver Health Medical Center

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Dit rapport beschrijft technieken om gesulfateerde glycosaminoglycanen (GAG's) uit biologische monsters te isoleren en te zuiveren en een polyacrylamidegel-elektroforesebenadering om hun grootte te benaderen. GAG's dragen bij aan de weefselstructuur en beïnvloeden signaleringsprocessen via elektrostatische interactie met eiwitten. GAG-polymeerlengte draagt bij aan hun bindingsaffiniteit voor verwante liganden.

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Biologie Nummer 168
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