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Preparing Lamellae from Vitreous Biological Samples Using a Dual-Beam Scanning Electron Microscope for Cryo-Electron Tomography
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Preparing Lamellae from Vitreous Biological Samples Using a Dual-Beam Scanning Electron Microscope for Cryo-Electron Tomography

Article DOI: 10.3791/62350-v • 07:00 min • August 5th, 2021
August 5th, 2021 •
1,2, 3,4, 3, 1, 1
1Centre for Ultrastructural Imaging, New Hunt’s House, Guy’s Campus, King’s College London, 2Department of Biological Science, Birkbeck College, University of London, 3Electron Bio-Imaging Centre, Diamond Light Source, Harwell Science and Innovation Campus, 4SLAC National Accelerator Laboratory, Stanford University

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Using focused ion beam milling to produce vitreous on-grid lamellae from plunge frozen biological samples for cryo-electron tomography.

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Preparing Lamellae Vitreous Biological Samples Dual-Beam Scanning Electron Microscope Cryo-Electron Tomography Plasmodium-falciparum-infected Red Blood Cells Structural Information Malaria Parasite Cryo-electron Tomography Protein Complexes Three-dimensional Structural Information Cell Types Frozen Copper Grids CryoStage Light Microscope Ice Gradient Cell Coverage Cryo-FIB-SEM-specific Autogrid Rims Carbon Or Platinum E-beam Rotary Coat Cell Distribution
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