Waiting
Processando Login

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / The Use of the Patch-Clamp Technique to Study the …
JoVE Journal
Biology

É necessário ter uma assinatura JoVE para assistir este

JoVE Journal Biology
The Use of the Patch-Clamp Technique to Study the Thermogenic Capacity of Mitochondria
Detection of Protein Aggregation using Fluorescence Correlation Spectroscopy
Journal (Biology)
Detection of Protein Aggregation using Fluorescence Correlation Spectroscopy
Workflow for High-content, Individual Cell Quantification of Fluorescent Markers from Universal Microscope Data, Supported by Open Source Software
Journal (Biology)
Workflow for High-content, Individual Cell Quantification of Fluorescent Markers from Universal Microscope Data, Supported by Open Source Software
 

The Use of the Patch-Clamp Technique to Study the Thermogenic Capacity of Mitochondria

Article DOI: 10.3791/62618-v • 11:05 min • May 3rd, 2021
May 3rd, 2021 •
1
1Department of Physiology, David Geffen School of Medicine, University of California Los Angeles

Capítulos

Resumo
Automatic Translation

This method article details the main steps in measuring H+ leak across the inner mitochondrial membrane with the patch-clamp technique, a new approach to study the thermogenic capacity of mitochondria.

Tags

Patch-clamp Technique Thermogenic Capacity Mitochondria Proton Leak Inner Mitochondrial Membrane Molecular Mechanisms Conductances Calcium Metabolites Adenine Nucleotides Euthanized Mouse Alcohol Spray Incision Thorax Heart Dissection Isolation Solution Isolation Buffer Tissue Homogenization
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter