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JoVE Journal Immunology and Infection
Macrophage Differentiation and Polarization into an M2-Like Phenotype using a Human Monocyte-Like THP-1 Leukemia Cell Line
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Macrophage Differentiation and Polarization into an M2-Like Phenotype using a Human Monocyte-Like THP-1 Leukemia Cell Line

Article DOI: 10.3791/62652-v • 06:38 min • August 2nd, 2021
August 2nd, 2021 •
1, 1, 1, 1, 1
1Price Institute of Surgical Research, Department of Surgery, University of Louisville

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M2-like tumor-associated macrophages (TAM) are associated with tumor progression and poor prognosis in cancer. This protocol serves as a detailed guide to reproducibly differentiate and polarize THP-1 monocyte-like cells into M2-like macrophages within 14 days. This model is the basis to investigate the anti-inflammatory effects of TAM within the tumor microenvironment.

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Macrophage Differentiation Polarization M2-like Phenotype THP-1 Leukemia Cell Line Anti-inflammatory Tumor Associated Macrophages Cancer Progression Poor Prognosis Protocol Differentiation Guide THP-1 Monocyte-like Cells M2-like Macrophages Anti-inflammatory THP-1 Polarization Resting Periods Prolonged Polarization Process Distinct M2-like Macrophage Subtype Tumor Development Colon Cancer Tissue Remodeling Reproducible Findings Cell Thawing Process
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