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In vivo Assessment of Microtubule Dynamics and Orientation in Caenorhabditis elegans Neurons
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Neurociência
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JoVE Journal Neurociência
In vivo Assessment of Microtubule Dynamics and Orientation in Caenorhabditis elegans Neurons

In vivo Assessment of Microtubule Dynamics and Orientation in Caenorhabditis elegans Neurons

DOI:
10.3791/62744-v

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07:43 min

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November 20, 2021

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,
1DBT-National Brain Research Centre

Summary

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A protocol for imaging the dynamic microtubules in vivo using fluorescently labeled End binding protein has been presented. We described the methods to label, image, and analyze the dynamic microtubules in the Posterior lateral microtubule (PLM) neuron of C. elegans.

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Microtubule DynamicsMicrotubule OrientationCaenorhabditis Elegans NeuronsIn Vivo AssessmentCytoskeletonAlpha And Beta TubulinEnd-binding ProteinsEBP-2GFPPLM NeuronsMake-3 PromoterMountingPolystyrene BeadsFluorescence MicroscopySpinning Disk Unit

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