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Generation af naive Blastoderm Explants fra Zebrafish Embryoner

Article DOI: 10.3791/62797-v • 07:21 min • July 30th, 2021
July 30th, 2021 •
1, 1
1Center for Precision Environmental Health and Department of Molecular and Cellular Biology, Baylor College of Medicine

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Zebrafisk blastoderm explants genereres ved at isolere embryonale celler fra endogene signalcentre i det tidlige embryo, der producerer relativt naive celle klynger let manipuleret og kultiveret ex vivo. Denne artikel indeholder instruktioner til fremstilling af sådanne explants og demonstrerer deres nytte ved at forhøre roller for Nodal signalering under gastrulation.

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Udviklingsbiologi udgave 173
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