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Isolation of Human Neutrophils from Whole Blood and Buffy Coats
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Isolation of Human Neutrophils from Whole Blood and Buffy Coats

Article DOI: 10.3791/62837-v • 08:12 min • September 17th, 2021
September 17th, 2021 •
1, 1, 1, 1,2
1Department of Pathology, Harvard Medical School, Department of Lab Medicine, Children’s Hospital Boston, and Dana-Farber/Harvard Cancer Center, 2Department of Microbiology, Faculty of Science, Mahidol University

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This protocol details a method for the isolation of neutrophils from whole blood, buffy coats, or leukapheresis membranes, achieving good yield, high purity, and minimal cell activation. We utilize gradient purification, red blood cell (RBC) sedimentation, and RBC lysis to obtain a high-quality/purity neutrophil preparation.

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Isolation Human Neutrophils Whole Blood Buffy Coats Cell Lines Neutrophil Biology Pure Neutrophils Healthy Neutrophils Fresh Neutrophils Density Gradient Sedimentation Gentle Lysis Neutrophilic Preparation Sterilizing Laminar Hood Blood Dilution Gradient Layering Red Blood Cells Density Gradient Medium Spinning
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