Waiting
Processando Login

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Neuroscience / Combined Mechanical and Enzymatic Dissociation of …
JoVE Journal
Neuroscience

É necessário ter uma assinatura JoVE para assistir este

JoVE Journal Neuroscience
Combined Mechanical and Enzymatic Dissociation of Mouse Brain Hippocampal Tissue
Measuring Circadian and Acute Light Responses in Mice using Wheel Running Activity
Journal (Neuroscience)
Measuring Circadian and Acute Light Responses in Mice using Wheel Running Activity
Quantification of Endosome and Lysosome Motilities in Cultured Neurons Using Fluorescent Probes
Journal (Neuroscience)
Quantification of Endosome and Lysosome Motilities in Cultured Neurons Using Fluorescent Probes
 

Combined Mechanical and Enzymatic Dissociation of Mouse Brain Hippocampal Tissue

Article DOI: 10.3791/63007-v • 07:14 min • October 21st, 2021
October 21st, 2021 •
1,2,3, 1,2,3, 1,2,3, 1,2, 1,2,3, 1,2,3
1Division of Radiation Health, University of Arkansas for Medical Sciences, 2Department of Pharmaceutical Sciences, University of Arkansas for Medical Sciences, 3Neurobiology & Developmental Sciences, University of Arkansas for Medical Sciences

Capítulos

Resumo
Automatic Translation

This neural cell dissociation protocol is intended for samples with a low amount of starting material and yields a highly viable single-cell suspension for downstream analysis, with optional fixation and staining steps.

Tags

Mechanical And Enzymatic Dissociation Mouse Brain Hippocampal Tissue Neural Tissue Treatment Efficacy Cellular Function Disease Mechanisms Treatment Mechanisms Single-cell Suspension Protocol Commercial Kits Planning Preparation Anesthetizing C57BL/6J Mouse Lower Abdomen Forceps Scissors Fur And Skin Ribcage Diaphragm Heart Connective Tissue Right Atrium Isoflurane Flow Breather Butterfly Needle Left Ventricle Pump Saline Solution
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter