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Intratibial Osteosarcoma Cell Injection to Generate Orthotopic Osteosarcoma and Lung Metastasis Mouse Models

Article DOI: 10.3791/63072-v • 04:25 min • October 28th, 2021
October 28th, 2021 •
*1,2, *1,2, 1,2, 1,2, 1,2, 1,2
1Longhua Hospital, Shanghai University of Traditional Chinese Medicine, 2Key Laboratory of theory and therapy of muscles and bones, Ministry of Education
* These authors contributed equally

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The present protocol describes intratibia osteosarcoma cell injection to generate mouse models bearing orthotopic osteosarcoma and pulmonary metastasis lesions.

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Intratibial Injection Osteosarcoma Cell Mouse Model Orthotopic Osteosarcoma Lung Metastasis Tumor Formation Rates Tumor Volumes Trypsinize Cell Concentration Automatic Cell Counter Centrifuge Cell Pellet Basement Membrane Matrix Injection Site Tibia
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