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Quantification of Vascular Parameters in Whole Mount Retinas of Mice with Non-Proliferative and Proliferative Retinopathies

Article DOI: 10.3791/63126-v • 12:28 min • March 12th, 2022
March 12th, 2022 •
1,2, 1,2,4, 1,2, 3, 1,2, 1,2
1Departamento de Bioquímica Clínica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, 2Centro de Investigaciones en Bioquímica Clínica e Inmunología (CIBICI), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), 3Vitreo-retinal Department, Centro Privado de Ojos Romagosa-Fundación VER, 4Unidad de Investigación y Desarrollo en Tecnología Farmacéutica (UNITEFA), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET)

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This article describes a well-established and reproducible lectin stain assay for the whole mount retinal preparations and the protocols required for the quantitative measurement of vascular parameters frequently altered in proliferative and non-proliferative retinopathies.

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Vascular Parameters Whole Mount Retinas Mice Non-proliferative Retinopathy Proliferative Retinopathy Vascular Alterations Disease Progression Treatment Choice Animal Models Oxygen-Induced Retinopathy Mouse Model Retinopathy Of Prematurity Proliferative Diabetic Retinopathy Avascular Areas Neovascular Areas Arteriole Dilatation Arteriole Tortuosity Metabolic Syndrome Mouse Model Nonproliferative Retinopathy Vascular Density Branching Evaluation Enucleatize With Scissors Paraformaldehyde Fixation Cornea Removal Retina Dissection RPE-choroid Separation
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