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Deep-Tissue Three-Photon Fluorescence Microscopy in Intact Mouse and Zebrafish Brain

Article DOI: 10.3791/63213-v • 08:26 min • January 13th, 2022
January 13th, 2022 •
*1, *1, 2, 1,3, 1,4, 1, 1, 1, 2, 1
1School of Applied and Engineering Physics, Cornell University, 2Department of Neurobiology and Behavior, Cornell University, 3College of Veterinary Medicine, Cornell University, 4Meinig School of Biomedical Engineering, Cornell University
* These authors contributed equally

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Three-photon microscopy enables high-contrast fluorescence imaging deep in living biological tissues, such as mouse and zebrafish brains, with high spatiotemporal resolution.

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Deep-tissue Three-photon Fluorescence Microscopy In Vivo Imaging Mouse Brain Zebrafish Brain Life Sciences High-spatial Resolution Imaging Neurological Diseases Alzheimer's Autism Brain Imaging Procedure Kibaek Choe Kristine Kolkman Laser Idler Output Optical Parametric Amplifier Pulse Compressors Femtosecond Laser Brewster's Angle Silicon Plate Flipper Mirrors
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