Waiting
Processando Login

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Developmental Biology / Differentiation of Human Pluripotent Stem Cells…
JoVE Journal
Developmental Biology

É necessário ter uma assinatura JoVE para assistir este

JoVE Journal Developmental Biology
Differentiation of Human Pluripotent Stem Cells Into Pancreatic Beta-Cell Precursors in a 2D Culture System
Automated Cell Culture System Settings for Maintenance and Differentiation of Human Pluripotent Stem Cells (Video) | JoVE
Journal (Developmental Biology)
Automated Cell Culture System Settings for Maintenance and Differentiation of Human Pluripotent Stem Cells (Video) | JoVE
Generation of Genetically Modified Mice through the Microinjection of Oocytes
Journal (Developmental Biology)
Generation of Genetically Modified Mice through the Microinjection of Oocytes
 

Differentiation of Human Pluripotent Stem Cells Into Pancreatic Beta-Cell Precursors in a 2D Culture System

Article DOI: 10.3791/63298-v • 10:12 min • December 16th, 2021
December 16th, 2021 •
1,2, 1,2
1College of Health and Life Sciences, Hamad Bin Khalifa University (HBKU), Qatar Foundation, 2Diabetes Research Center, Qatar Biomedical Research Institute (QBRI), Hamad Bin Khalifa University (HBKU), Qatar Foundation (QF)

Capítulos

Resumo
Automatic Translation

The present protocol describes an enhanced method to increase the co-expression of PDX1 and NKX6.1 transcription factors in pancreatic progenitors derived from human pluripotent stem cells (hPSCs) in planar monolayers. This is achieved by replenishing the fresh matrix, manipulating cell density, and dissociating the endodermal cells.

Tags

Differentiation Human Pluripotent Stem Cells Pancreatic Beta-cell Precursors 2D Culture System Cell Therapy Diabetes Molecular Mechanisms Monolayer Cultures Insulin Secretion Hyperglycemia Pancreatic Progenitors Tissue Samples Early Pancreatic Development HPSC Colonies PBS Differentiation Medium
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter