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Quantifying the Binding Interactions Between Cu(II) and Peptide Residues in the Presence and Absence of Chromophores
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Quantifying the Binding Interactions Between Cu(II) and Peptide Residues in the Presence and Absence of Chromophores

Article DOI: 10.3791/63668-v • 11:38 min • April 5th, 2022
April 5th, 2022 •
1, 2, 2, 1
1Department of Chemistry, University of San Francisco, 2Department of Chemistry, University of California, Davis

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This article focuses on the use of electronic absorption spectroscopy and isothermal titration calorimetry to probe and quantify the thermodynamics of Cu(II) binding to peptides and proteins.

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Quantifying Binding Interactions Cu(II) Peptide Residues Presence Absence Chromophores Researcher Hard To Study Systems Orthogonal Techniques Insight Phenomenon Video Method Metal Peptide Interactions Metal Protein Interactions Buffer Choice Literature Review Demonstration Procedure Sohee Choi Graduate Student Electronic Absorption Spectrophotometer Spectrophotometer Software Scanning Range Nanometers Per Second Double Beam Baseline Corrected Matched Cuvettes Ultrapure Water
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