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Neuroscience

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JoVE Journal Neuroscience
Full- versus Sub-Regional Quantification of Amyloid-Beta Load on Mouse Brain Sections
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Full- versus Sub-Regional Quantification of Amyloid-Beta Load on Mouse Brain Sections

Article DOI: 10.3791/63669-v • 07:28 min • May 19th, 2022
May 19th, 2022 •
1, 2, 3, 4, 4,5
1Henry E. Riggs School of Applied Life Sciences, Keck Graduate Institute, 2Crean College of Health and Behavioral Sciences, Chapman University, 3Keck Science Department, Claremont McKenna College, 4Department of Biomedical and Pharmaceutical Sciences, School of Pharmacy, Chapman University, 5Department of Neurology, University of California, Irvine

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The present protocol describes and compares the procedure to perform a full-region or sub-region of interest analysis of sagittal mouse brain sections to quantify amyloid-beta load in the APP/PS1 transgenic mouse model of Alzheimer's disease.

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Amyloid-beta Load Mouse Brain Sections Full Sections Sub-regions APP/PS1 Transgenic Mice Image Analysis Software Straight Tool Scale Bar Analyze Set Scale Measure Pixels Micrometer Global Box Area Measurement Hippocampus Cortex Visualization Brightness Adjustment
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