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Neuroscience

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JoVE Journal Neuroscience
Robust and Highly Reproducible Generation of Cortical Brain Organoids for Modelling Brain Neuronal Senescence In Vitro
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Robust and Highly Reproducible Generation of Cortical Brain Organoids for Modelling Brain Neuronal Senescence In Vitro

Article DOI: 10.3791/63714-v • 05:40 min • May 5th, 2022
May 5th, 2022 •
*1, *1, 1
1Australian Institute for Bioengineering and Nanotechnology, The University of Queensland
* These authors contributed equally

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In this study, we provide a detailed technique for a simple yet robust cortical organoid culture system using standard feeder-free hPSC cultures. This is a rapid, efficient, and reproducible protocol for generating organoids that model aspects of brain senescence in vitro.

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Cortical Brain Organoids Neuronal Senescence In Vitro Modeling Robustness Reproducibility HPSCs Neuroectodermal Colonies Neuroepithelial Spheroids Tissue Architecture Senescence Markers Aging-related Neuronal Processes N2 Medium Dual SMAD Inhibitors 2D To 3D Conversion Dispase Colony Detachment
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