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Analysis of Cardiac Contractile Dysfunction and Ca2+ Transients in Rodent Myocytes
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Analysis of Cardiac Contractile Dysfunction and Ca2+ Transients in Rodent Myocytes

Article DOI: 10.3791/64055-v • 07:32 min • May 25th, 2022
May 25th, 2022 •
1, 1
1Department of Cardiac Surgery, Michigan Medicine, University of Michigan, Ann Arbor

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A set of protocols are presented that describe the measurement of contractile function via sarcomere length detection along with calcium (Ca2+) transient measurement in isolated rat myocytes. The application of this approach for studies in animal models of heart failure is also included.

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Cardiac Contractile Dysfunction Ca2+ Transients Rodent Myocytes Heart Failure Neurohormones Therapeutic Agents Sarcomere Length Imaging Gel Pack Media Incubator Contractile Function Platform Tubing Peristaltic Pump Cell Stimulator Perfusion Cover Slip Chamber Platinum Electrode Mount
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