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Organotypic Retinal Explant Cultures from Macaque Monkey

Article DOI: 10.3791/64178-v • 10:32 min • August 24th, 2022
August 24th, 2022 •
1, 1, 1, 1, 2, 1
1Yunnan Eye Institute & Key Laboratory of Yunnan Province, Yunnan Eye Disease Clinical Medical Center, Affiliated Hospital of Yunnan University, Yunnan University, 2Institute for Ophthalmic Research, Eberhard-Karls-Universität Tübingen

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Retinal explants obtained from wild-type macaques were cultured in vitro. Retinal degeneration and the cGMP-PKG signaling pathway was induced using the PDE6 inhibitor zaprinast. cGMP accumulation in the explants at different zaprinast concentrations was verified using immunofluorescence.

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Organotypic Retinal Explant Cultures Macaque Monkey CGMP-PKG Dependent Retinal Deterioration Nonhuman Primate Model Intercellular Interactions Experimental Duration Retinal Development Retinal Degeneration Wild Type Macaques Povidone Iodine Penicillin Streptomycin PBS Solution R16 Medium Protein Ace-K Solution Fetal Bovine Serum Solution Sclera Cornea Iris Lens Vitreous Body
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