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Bioengineering

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JoVE Journal Bioengineering
Reconstitution of Actin-Based Motility with Commercially Available Proteins
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Reconstitution of Actin-Based Motility with Commercially Available Proteins

Article DOI: 10.3791/64261-v • 08:40 min • October 28th, 2022
October 28th, 2022 •
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1Laboratoire de physique de l’Ecole Normale Supérieure, ENS, Université PSL, CNRS, Sorbonne Université, Université Paris Cité

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This protocol describes how to produce actin comets on the surfaces of beads using commercially available protein ingredients. Such systems mimic the protrusive structures found in cells, and can be used to examine physiological mechanisms of force production in a simplified way.

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Actin-based Motility Proteins Functionalized Surfaces Ingredient Mix Force Production Protein Purification Undergraduate-level Teaching Module Hands-on Experience Microscope Observation System Parameters Physical Analysis Langmuir Equation Lyophilized Proteins Water Incubation Depolymerize Actin Oligomers G-buffer
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