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The Isolation of Flowing Mesenteric Lymph in Mice to Quantify In Vivo Kinetics of Dietary Lipid Absorption and Chylomicron Secretion

Article DOI: 10.3791/64338-v • 06:14 min • November 30th, 2022
November 30th, 2022 •
1, 1, 1
1Division of Endocrinology and Metabolism, Department of Medicine, School of Medicine, University of Pittsburgh

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The present protocol describes a detailed surgical protocol for isolating flowing intestinal lymph in response to intraduodenal nutrient infusions in mice. This allows for the physiological determination of total intestinal lipid absorption and chylomicron synthesis and secretion in response to various experimental nutrients.

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Flowing Mesenteric Lymph In Vivo Kinetics Dietary Lipid Absorption Chylomicron Secretion Postprandial Metabolism Small Intestine Lymphocytes Cytokines Dietary Lipids Chylomicrons Synthesis And Secretion One Day Model Experimental Time Reduction Animal Stress Reduction Animal Survival Increase Experimental Number Decrease Surgery Preparation Abdominal Incision Peritoneal Viscera Lymph Duct Exposure Superior Mesenteric Artery Intestinal Lymph Duct Stretching Cannula Tubing
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