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Live-Cell Imaging of Intact Ex Vivo Globes Using a Novel 3D Printed Holder
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Live-Cell Imaging of Intact Ex Vivo Globes Using a Novel 3D Printed Holder

Article DOI: 10.3791/64510-v • 05:41 min • October 6th, 2022
October 6th, 2022 •
*1, *2, 2, 3, 2,3
1Department of Pharmacology, School of Medicine, Boston University, 2Department of Biochemistry, School of Medicine, Boston University, 3Department of Ophthalmology, School of Medicine, Boston University
* These authors contributed equally

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Resumo
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The present work describes a novel experimental protocol that utilizes a 3D printed holder to enable high-resolution live cell imaging of enucleated globes. Through this protocol, the cellular calcium signaling activity in wounded corneal epithelium from ex vivo globes can be observed in real time.

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Live-cell Imaging 3D Printed Holder Ex Vivo Globes Cell Signaling Cellular Migration Wounded Corneas Real Time Monitoring High Quality Live Images Corneal Cells Epithelium Nerves Globe Modification Nano Indentation Experiments Euthanized Mouse Enucleation Calcium Indicator Cell Membrane Stain Incubator Staining Medium Glass Bottom Cover Slip
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