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Isolatie van rustige stamcelpopulaties van individuele skeletspieren
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Isolatie van rustige stamcelpopulaties van individuele skeletspieren

Article DOI: 10.3791/64557-v • 11:35 min • December 9th, 2022
December 9th, 2022 •
*1, *1, 1, 1, 1
1Department of Biomedicine, Aarhus University
* These authors contributed equally

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Dit protocol beschrijft de isolatie van spierstamcellen en fibro-adipogene voorlopers uit individuele skeletspieren bij muizen. Het protocol omvat enkelvoudige spierdissectie, stamcelisolatie door fluorescentie-geactiveerde celsortering, zuiverheidsbeoordeling door immunofluorescentiekleuring en kwantitatieve meting van S-fase-invoer door 5-ethynyl-2'-deoxyuridine-incorporatietest.

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