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JoVE Journal Neuroscience
Robust Tissue Fabrication for Long-Term Culture of iPSC-Derived Brain Organoids for Aging Research
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Robust Tissue Fabrication for Long-Term Culture of iPSC-Derived Brain Organoids for Aging Research

Article DOI: 10.3791/64586-v • 08:20 min • May 12th, 2023
May 12th, 2023 •
1, 2,3, 1
1Department of Applied Stem Cell Technologies, TechMed Centre Enschede, University of Twente, 2Centro de Biotecnología-FEMSA, Tecnologico de Monterrey, 3Department of Genetics, Harvard Medical School

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The present protocol provides a step-by-step procedure for the reproducible generation, maintenance, and aging of cerebral organoids derived from human-induced pluripotent stem cells (iPSCs). This method enables culturing and maturing cerebral organoids for extended periods, which facilitates the modeling of processes involved in brain aging and age-related pathogenesis.

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Tissue Fabrication Long-term Culture IPSC-derived Brain Organoids Aging Research Protocol Techniques Age-related Diseases Clinical Manifestations Specialized Equipment Reagents Lab Materials Neurodegenerative Diseases Embryonic Stem Cells IPSCs Delicate Cells First-time Users Patience 70% Ethanol Spray PLGA Microfilaments Suture Strand Blunt End Of Scalpel Microscope Ruler Cutting Fiber Strands Antibiotic-Antimycotic Solution DMEM/F-12 Dilution 96-well Plate
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