Simple Continuous Glucose Monitoring in Freely Moving Mice

Doron Kleiman; Mika Littor; Mahmoud Nawas; Rachel Ben-Haroush Schyr; Danny Ben-Zvi

doi:10.3791/64743

Monitoramento Contínuo Simples de Glicose em Camundongos em Movimento Livre

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JoVE Journal Biology

Simple Continuous Glucose Monitoring in Freely Moving Mice

Monitoramento Contínuo Simples de Glicose em Camundongos em Movimento Livre

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03:25 min

February 24, 2023

DOI: 10.3791/64743-v

Doron Kleiman¹, Mika Littor^1,2, Mahmoud Nawas³, Rachel Ben-Haroush Schyr¹, Danny Ben-Zvi¹

¹Department of Developmental Biology and Cancer Research, Institute of Medical Research Israel-Canada,The Hebrew University-Hadassah Medical School, ²Department of Military Medicine and “Tzameret”, Faculty of Medicine,Hebrew University of Jerusalem, and Medical Corps, Israel Defense Forces, ³Department of Surgery,Hadassah Medical Center-Ein Kerem Campus

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Overview

This study presents a straightforward method for implanting a commercial continuous glucose monitor (CGM) in mice to track glucose levels. The technique offers significant advantages, eliminating the need for complex microsurgery or bleeding, thus facilitating studies in obesity and diabetes models.

Key Study Components

Research Area

Diabetes research
Metabolic phenotype identification
Glucose monitoring in animal models

Background

Importance of monitoring glucose levels in obesity and diabetes research
Challenges associated with traditional surgical approaches
Need for effective methods to explore glucose management phenotypes

Methods Used

The procedure for inserting a CGM cannula
Mice as the biological model system
Utilization of suture techniques and sensor activation

Main Results

The method successfully secured the CGM cannula for extended monitoring
Data collected revealed new insights into metabolic phenotypes
Demonstrated feasibility and efficiency in data acquisition from mice

Conclusions

The study illustrates a reliable protocol for glucose monitoring in mice
This method contributes to advancing research in metabolic disorders

Frequently Asked Questions

What is the main advantage of this glucose monitoring method?

It simplifies the monitoring process and eliminates the need for invasive surgery or bleeding, making it less stressful for the mice.

How long can the CGM monitor glucose levels?

The method allows for continuous data collection, with effective monitoring over multiple days, as demonstrated in a three-day data collection period.

What biological models can benefit from this technique?

The technique is particularly useful in mouse models of obesity and diabetes, aiding in the identification of glucose management phenotypes.

What preparations are necessary before implanting the CGM?

It involves shaving the midline of the mouse's back, using depilatory cream, and making a small incision for cannula placement.

Are there any specific challenges when implementing this method?

The critical step is the careful insertion of the cannula and securing it properly to ensure it remains in place during the monitoring period.

Can the data collection parameters be adjusted?

Yes, the scripts provided allow modulation of various parameters during data analysis.

What type of data does this method produce?

The method produces continuous glucose monitoring data, which can reveal metabolic insights in mouse models.

Aqui, descrevemos um método simples para implantar um monitor de glicose contínuo comercial projetado para pacientes em camundongos e fornecemos os scripts para analisar os resultados.

Nosso protocolo oferece um método simples e barato para monitorar os níveis de glicose em camundongos. A principal vantagem dessa técnica é que ela não requer microcirurgia desafiadora, e não envolve sangramento ou manuseio dos camundongos. Este método pode ser usado para identificar fenótipos elusivos de controle glicêmico em modelos murinos de obesidade e diabetes.

Durante a tentativa desse protocolo, o passo mais crítico é a inserção da cânula sob a pele e o aperto das suturas internas para manter a cânula no lugar. Para começar, raspe a linha média da parte de trás do rato anestesiado e use um creme depilatório para garantir a depilação completa. Abra e carregue o sensor.

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