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Contact Mode Atomic Force Microscopy as a Rapid Technique for Morphological Observation and Bacterial Cell Damage Analysis

Article DOI: 10.3791/64823-v • 05:34 min • June 30th, 2023
June 30th, 2023 •
1, 1, 1, 2, 1
1Centro Universitario de los Lagos, Universidad de Guadalajara, 2Centro Nacional de Recursos Genéticos, Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias

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Here, we present the application of atomic force microscopy (AFM) as a simple and fast method for bacterial characterization and analyze details such as the bacterial size and shape, bacterial culture biofilms, and the activity of nanoparticles as bactericides.

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Contact Mode Atomic Force Microscopy Morphological Observation Bacterial Cell Damage Analysis Sealed Containers Fixed Sample Flame Fixation Petri Dish Atomic Force Microscope Equipment Computer Software ContAI-G Probes Auto Slope Options Z Controller Set Point P Gain I Gain D Gain Tip Voltage Scan Range Head AFM Contact Mode Camera Mounted On Lenses XY Plane Displacement Electronic Approach XY Measurement Plane Adjustment
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