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Generation and Maintenance of Primate Induced Pluripotent Stem Cells Derived from Urine

Article DOI: 10.3791/64922-v • 07:46 min • July 28th, 2023
July 28th, 2023 •
1, 1, 1, 1, 1,2,3
1Faculty of Biology, Ludwig Maximilian University of Munich, 2Institute for the Advanced Study of Human Biology, Kyoto University, 3Hakubi Center, Kyoto University

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The present protocol describes a method to isolate, expand, and reprogram human and non-human primate urine-derived cells to induced pluripotent stem cells (iPSCs), as well as instructions for feeder-free maintenance of the newly generated iPSCs.

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Primate Induced Pluripotent Stem Cells Urine-derived IPSCs Generation Protocol Maintenance Protocol Non-invasive Cell Source Centrifugation Urine Wash Buffer Amphotericin Gelatin-coated Plate Basement Membrane Matrix-coated Plate
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