Scalable Transfection of Maize Mesophyll Protoplasts

Jackson Tonnies; Nicholas A. Mueth; Sayeh Gorjifard; Jonah Chu; Christine Queitsch

doi:10.3791/64991

Journal / Biology /

JoVE Journal
Biology
Author Produced

Este � de .

JoVE Journal Biology

Scalable Transfection of Maize Mesophyll Protoplasts

Journal (Biology)
Combined Nucleotide and Protein Extractions in Caenorhabditis elegans

Journal (Biology)
C. elegans Chemotaxis Assay

The JoVE video player is compatible with HTML5 and Adobe Flash. Older browsers that do not support HTML5 and the H.264 video codec will still use a Flash-based video player. We recommend downloading the newest version of Flash here, but we support all versions 10 and above.

If that doesn't help, please let us know.

Unable to load video. Please check your Internet connection and reload this page. If the problem continues, please let us know and we'll try to help.

An unexpected error occurred. Please check your Internet connection and reload this page. If the problem continues, please let us know and we'll try to help.

Scalable Transfection of Maize Mesophyll Protoplasts

Article DOI: 10.3791/64991-v • 08:38 min • June 23rd, 2023
June 23rd, 2023 •

Jackson Tonnies¹, Nicholas A. Mueth¹, Sayeh Gorjifard¹, Jonah Chu¹, Christine Queitsch¹

¹Genome Sciences Department, University of Washington

Capítulos

Resumo
Automatic Translation

العربية (Arabic)
中文 (Chinese)
dansk (Danish)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
हिंदी (Hindi)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
norsk (Norwegian)
português (Portugese)
русский (Russian)
español (Spanish)
svenska (Swedish)
Türkçe (Turkish)

Here, we present a high-throughput protocol to transiently transfect millions of maize protoplasts for testing large libraries of plasmids in maize mesophyll cells.

Tags
Scalable Transfection Maize Mesophyll Protoplasts Protocol Plant Cell Wall Digestion PEG Transformation High Efficiency Millions Of Transformed Protoplasts Maize Seedlings Dark Growth Leaf Selection Leaf Cutting Consistent Thin Slices Enzyme Solution

Artigo Incorporar

ADICIONAR À PLAYLIST Estatísticas de uso

https://www.jove.com/t/64991

Vídeos Relacionados

Fluorescence Activated Cell Sorting of Plant Protoplasts

Fluorescence Activated Cell Sorting of Plant Protoplasts

Investigating Fungal Pathogenicity Mechanisms in Maize (Video) | JoVE

Investigating Fungal Pathogenicity Mechanisms in Maize (Video) | JoVE

ï»¿Imaging Transverse Sections of Maize Leaf Primordia (Video) | JoVE

ï»¿Imaging Transverse Sections of Maize Leaf Primordia (Video) | JoVE

Imaging Unrolled Whole Mounts of Maize Leaf Primordia (Video) | JoVE

Imaging Unrolled Whole Mounts of Maize Leaf Primordia (Video) | JoVE

Measuring Endoreduplication by Flow Cytometry of Isolated Tuber Protoplasts

Measuring Endoreduplication by Flow Cytometry of Isolated Tuber Protoplasts

Investigating Fungal Pathogenicity Mechanisms in Maize (Video) | JoVE

Investigating Fungal Pathogenicity Mechanisms in Maize (Video) | JoVE

Improved Methods for Preparing Transverse Sections and Unrolled Whole Mounts of …

Improved Methods for Preparing Transverse Sections and Unrolled Whole Mounts of …

Lateral Root Inducible System in Arabidopsis and Maize

Lateral Root Inducible System in Arabidopsis and Maize

Scalable, Flexible, and Cost-Effective Seedling Grafting

Scalable, Flexible, and Cost-Effective Seedling Grafting

A Highly Scalable Approach to Perform Ecological Surveys of Selfing Caenorhabditis Nematodes…

A Highly Scalable Approach to Perform Ecological Surveys of Selfing Caenorhabditis Nematodes…

Read Article

Journal (Biology)

Journal (Biology)

Scalable Transfection of Maize Mesophyll Protoplasts

Capítulos

Tags

Vídeos Relacionados

Get cutting-edge science videos from JoVE sent straight to your inbox every month.