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Adipocyte-Specific ATAC-Seq with Adipose Tissues Using Fluorescence-Activated Nucleus Sorting

Article DOI: 10.3791/65033-v • 11:11 min • March 17th, 2023
March 17th, 2023 •
1, 1, 1, 1, 1
1Department of Biochemistry and Molecular Biology, Indiana University School of Medicine

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We present a protocol for assay for transposase-accessible chromatin with high-throughput sequencing (ATAC-seq) specifically on adipocytes using nucleus sorting with adipose tissues isolated from transgenic reporter mice with nuclear fluorescence labeling.

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Adipocyte-Specific ATAC-Seq Adipose Tissues Fluorescence-Activated Nucleus Sorting Gene Expression Regulatory Mechanisms Lipid Contents Mitochondrial Contamination Cellular Heterogeneity Adipocyte-specific ATAC-sequencing High-quality Data Mitochondrial DNA Contamination Nucleus Isolation Glass Dounce Homogenizers NPB Mix Frozen Adipose Tissue Scissors Loose Pestle Tight Pestle 100 Micrometer Strainer 50 Milliliter Tube Centrifugation Supernatant PBS-N Solution 40 Micrometer Strainer
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