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Generation of 3D Tumor Spheroids for Drug Evaluation Studies
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Generation of 3D Tumor Spheroids for Drug Evaluation Studies

Article DOI: 10.3791/65125-v • 10:33 min • February 24th, 2023
February 24th, 2023 •
*1,2, *3,4, 3,4, 2,4, 4, 2,4, 2,3, 1,2
1Department of Oncology, School of Medicine, Southeast University, 2State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, 3Institute of Medical Devices (Suzhou), Southeast University, 4Jiangsu Avatarget Biotechnology Co., Ltd.
* These authors contributed equally

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This article demonstrates a standardized method for constructing three-dimensional tumor spheroids. A strategy for spheroid observation and image-based deep-learning analysis using an automated imaging system is also described.

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3D Tumor Spheroids Drug Evaluation Studies Standardized Method High Content Analysis High Throughput Imaging Drug Tests AMG510 NCI-H23 Spheroid Anti Adhesion Reagent U-shaped Well Bottom 48 Well Plate Sterilized PBS Incubator Microscope T25 Flask Culture Medium Trypsin EDTA Suspension
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