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Advancing Mitochondrial Research Â– mtHyper7 Biosensor for Subcellular Analysis (Video) | JoVE

Imaging of Mitochondrial Peroxide in Living Yeast Cells Using mtHyper7

Article DOI: 10.3791/65428-v • 02:28 min • June 2nd, 2023
June 2nd, 2023 •

Emily Jie-Ning Yang¹, Istvan R. Boldogh^1,2, Haojie Ji², Liza Pon^1,2, Theresa C. Swayne^1,2

¹Department of Pathology and Cell Biology, Columbia University Irving Medical Center, ²Confocal and Specialized Microscopy Shared Resource in the Herbert Irving Comprehensive Cancer Center, Columbia University Irving Medical Center

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MtHyPer7 ביוסנסור רציומטרי מי חמצן מיטוכונדריאליים תאי שמרים חיים תפקוד לקוי של המיטוכונדריה הפרעות נוירודגנרטיביות הפרעות שרירים ושלד סרטן הזדקנות ביוסנסור מקודד גנטית זעיר פולשני מי חמצן (H2O2) HyPer7 ממוקד מיטוכונדריה (mtHyPer7) חלבון פלואורסצנטי שעבר שינוי מעגלי תחום חלבון OxyR מערכת CRISPR-Cas9 מערכת ללא סמן שילוב גנום שמרים מערכת מיקרוסקופ קונפוקלי דיסק מסתובב אנליזה כמותית חמצון מתח מידע מרחבי-זמני

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Journal (Biology)

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Advancing Mitochondrial Research Â– mtHyper7 Biosensor for Subcellular Analysis (Video) | JoVE

Imaging of Mitochondrial Peroxide in Living Yeast Cells Using mtHyper7

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