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Journal / Genetics / Dubbele immunofluorescentie van γH2AX en 53BP1 in …
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Genetics
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JoVE Journal Genetics
Dubbele immunofluorescentie van γH2AX en 53BP1 in humane perifere lymfocyten
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Dubbele immunofluorescentie van γH2AX en 53BP1 in humane perifere lymfocyten

Article DOI: 10.3791/65472-v • 05:34 min • July 14th, 2023
July 14th, 2023 •
1, 1,2, 1, 1
1Unità di Genetica, Dipartimento di Biologia, University of Pisa, 2Department of Women-Child-Newborn Obstetrics and Gynaecology, Foundation IRCCS Ca' Granda Ospedale Maggiore Policlinico

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Dit protocol presenteert een methode om de vorming en reparatie van DNA-dubbelstrengsbreuken te beoordelen door de gelijktijdige detectie van γH2AX- en 53BP1-foci in interfasekernen van met bleomycine behandelde menselijke perifere lymfocyten.

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Deze maand in JoVE nummer 197
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