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Journal / Genetics / Dobbel immunfluorescens av γH2AX og 53BP1 i…
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JoVE Journal Genetics
Dobbel immunfluorescens av γH2AX og 53BP1 i humane perifere lymfocytter
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Dobbel immunfluorescens av γH2AX og 53BP1 i humane perifere lymfocytter

Article DOI: 10.3791/65472-v • 05:34 min • July 14th, 2023
July 14th, 2023 •
1, 1,2, 1, 1
1Unità di Genetica, Dipartimento di Biologia, University of Pisa, 2Department of Women-Child-Newborn Obstetrics and Gynaecology, Foundation IRCCS Ca' Granda Ospedale Maggiore Policlinico

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Denne protokollen presenterer en metode for å vurdere dannelsen og reparasjonen av DNA-dobbeltstrengbrudd gjennom samtidig påvisning av γH2AX og 53BP1-foci i interfasekjerner av bleomycinbehandlede humane perifere lymfocytter.

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