Isolation, In Vitro Expansion, and Characterization of Mesenchymal Stem Cells from Mouse Epididymal Adipose Tissue

Marianna Gabrielle Almeida Galv&#227;o; Brisa Miranda Andrade Santos; Cah&#234; Moreira Aguiar; Adriana Bozzi

doi:10.3791/65722

Isolamento, expansão in vitro e caracterização de células-tronco mesenquimais do tecido adiposo e...

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Isolation, In Vitro Expansion, and Characterization of Mesenchymal Stem Cells from Mouse Epididymal Adipose Tissue

Isolamento, expansão in vitro e caracterização de células-tronco mesenquimais do tecido adiposo epididimal de camundongos

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04:53 min

January 12, 2024

DOI: 10.3791/65722-v

Marianna Gabrielle Almeida Galvão¹, Brisa Miranda Andrade Santos¹, Cahê Moreira Aguiar², Adriana Bozzi^1,2

¹Laboratory of Immunobiology, Department of Biological Sciences,State University of Santa Cruz - UESC, ²Department of Medicine,Afya Medical Sciences College

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Overview

This study focuses on the extraction, cultivation, and characterization of adipose tissue-derived stem cells (ADSCs) from the epididymal adipose tissue of Swiss mice. The findings highlight ADSCs' potential as a therapeutic tool, particularly in managing inflammation associated with diseases like Schistosoma mansoni infection.

Key Study Components

Research Area

Adipose-derived mesenchymal stem cells
Inflammation control
Therapeutic applications in infection

Background

High potential of adipose tissue as a source of stem cells
Represents an abundant and accessible source
Defines a protocol for meticulous collection and processing

Methods Used

Isolation and culture of ADSCs
Swiss mice as the biological model
Characterization techniques including staining and morphological assessment

Main Results

Demonstrated ADSCs' multi-lineage differentiation potential
Effective control of granulomatous reactions in Schistosoma mansoni infection
Characterization confirmed morphology and potential of ADSCs

Conclusions

This study provides a reliable protocol for ADSC extraction and characterization
Highlights the therapeutic promise of ADSCs in inflammatory diseases

Frequently Asked Questions

What are adipose-derived stem cells?

Adipose-derived stem cells (ADSCs) are multipotent stem cells found in adipose tissue, capable of differentiating into various cell types.

How were the stem cells extracted in this study?

Stem cells were extracted from the epididymal adipose tissue of Swiss mice following aseptic techniques and specific processing methods.

What diseases could benefit from ADSC therapy?

Potential applications include treatment for inflammatory diseases and tissue regeneration.

What is the significance of this research?

This research fills a gap in the techniques for isolating ADSCs and demonstrates their therapeutic potential in a disease model.

What methodologies were used for characterization?

Techniques included morphological assessments and staining methods to evaluate differentiation potential.

Can ADSCs be used in combination with other therapies?

Yes, the study indicates that ADSCs can be combined with other treatments, such as praziquantel, to improve therapeutic outcomes.

O tecido adiposo é uma excelente fonte de células-tronco mesenquimais. Aqui, trazemos a extração, cultivo e caracterização passo a passo de células-tronco derivadas do tecido adiposo (ADSCs) do tecido adiposo epididimal de camundongos suíços.

Minha pesquisa se concentra em células-tronco mesenquimais derivadas do tecido adiposo como uma nova abordagem de tratamento associada ou não a outras terapias para melhorar a inflamação de várias doenças. Demonstramos que o uso de células-tronco mesenquimais adiposas em conjunto com o Praziquantel controlou significativamente a reação granulomatosa na infecção por Schistosoma mansoni, destacando os benefícios dessa abordagem de tratamento combinado. Nosso protocolo aborda a lacuna de pesquisa na coleta meticulosa de tecido adiposo epididimal sob condições assépticas, que o processamento físico envolve agitação vigorosa e identifica o momento ideal para a utilização de células-tronco derivadas do tecido adiposo.

Nosso protocolo oferece vantagens significativas, notadamente a disponibilidade abundante e a força potencial para o isolamento de células-tronco mesenquimais. Além disso, as células-tronco mesenquimais apresentam propriedades terapêuticas potentes, incluindo capacidades anti-inflamatórias e de regeneração tecidual.

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Palavras-chave: Células-tronco mesenquimais CTMs tecido adiposo tecido adiposo epididimal isolamento expansão in vitro caracterização citometria de fluxo diferenciação osteogênica diferenciação adipogênica diferenciação condrogênica terapia celular transplante alogênico