Name: Estudo Multimodal da Remodelação Cardiovascular Murina: Ultrassom Quadridimensional e Imagem por Espectrometria de Massas (Vídeo) | JoVE Journal
Uploaded: 2025-01-10T13:00:00.000+00:00
Duration: 9 min 43 s
Description: Explore a remodelação cardiovascular murina usando ultrassom 4D e espectrometria de massas. Este protocolo integra imagem funcional e molecular para analisar alterações biomecânicas e biomoleculares em tecidos cardíacos e vasculares.

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<meta charset="utf8"></head><body>Ultrassom 4D e imagem MALDI para análise cardíaca e vascular em camundongos

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</ol>

<table><tbody><tr><td>Ácido 2,5-dihidroxibenzóico (DHB)</td><td>Supelco,&nbsp; >99,0% (HPLC)</td><td>85707-10MG-F</td><td>DHB substância da matriz para MALDI-MS; https://www.sigmaaldrich.com/US/en/product/sigma/85707?cm_sp=Insite-_-wimsShippingEmailRecs_wims EmailAPI_wimsGruCrossEntropy-_-wimsEmailAPI10-3</td></tr><tr><td>9-aminoacridina (9AA)</td><td>Supelco,&nbsp; &ge; 99,5% (HPLC)</td> <td>92817-1G</td><td>substância da matriz de 9-aminoacridina para MALDI-MS; https://www.sigmaaldrich.com/US/en/product/sial/92817?srsltid=AfmBOooiQjQ4pWv_XxITkU 4Lkm0UnHXKekGS_ dFl7V40V9QLWoPpNLoc</td></tr><tr><td>Gel de ultrassom aquosônico</td><td>Parker Laboratories</td><td>Parker 01-02</td><td>Gel de ultrassom; https://www.parkerlabs.com/products/aquasonic-100-ultrasound-transmission-gel/</td></tr> <tr><td>frascos de Dewar de bancada</td><td>TermoCientífica&nbsp;</td><td>4150-2000</td><td>Recipiente para nitrogênio líquido; https://www.thermofisher.com/order/catalog/product/4150-4000?gclid=Cj0KCQjwpvK4BhDUARIsA DHt9sQVc2f-NxN04Nb5Mv F6TZ7GLHWWDEeqDYmEvtKJSQ YHDeVgZ9qylvYaAs27EALw_wcB & fonte=google_shopping& ISO_ CÓDIGO=us& LANG_CODE=pt-br& ef_id =Cj0KCQjwpvK4BhDUARIsADHt9 sQVc2f-NxN04Nb5MvF6TZ7GLHWWDE eqDYmEvtKJSQYHDeVgZ9qylvYa As27EALw_wcB:G:s& s_kwcid=AL!3652 !3!716188292869!!! g!2366243726129 !! 21787513085!171591181194& ev_chn =loja& cid=0se_gaw_30092024_ PBYTXL& source=google_shopping & ISO_CODE = nós & LANG_CODE= en& gad_source=1</td></tr><tr><td>Criostato</td><td>Leica Biosystems</td><td>Série CM https://www.leicabiosystems.com/us/histology-equipment/cryostats/</td><td></td></tr> <tr><td>Dessecador</td><td>VWR&nbsp;</td><td>89054-052</td><td>https://us.vwr.com/store/product/9104882/desiccator-plastic-ace-glass-incorporated</td></tr><tr><td>Epredia MX35 Premier Descartável de perfil baixo&nbsp; Microtomo Lâminas</td><td>Fisher Scientific</td><td>3052835</td><td>Lâmina de criostato; https://www.fishersci.com/shop/products/mx35-premier-disposable-low-profile-microtome-blades/3052835</td></tr><tr><td>Falcon 15 mL Tubos de centrífuga cônicos</td><td>Fisher Scientific</td><td>14-959-53A</td><td>; https://www.fishersci.com/shop/products/falcon-15ml-conical-centrifuge-tubes-5/1495953A?gclid=Cj0KCQjwpvK4BhDUARIsA DHt9sSBcy5n-lhShligJUOX5KKVGn0bt87 8AB2_muOD2PPTue1phpZgeqwa AqgiEALw_wcB& ef_id=Cj0KCQjw pvK4BhDUARIsADHt9sSBcy5n-lhS hligJUOX5KKVGn0bt878AB2_muO D2PPTue1phpZgeqwaAqgiEALw_ wcB:G:s& ppc_id=PLA_goog_20861 45674_81843405034_1495953A__ 386247001345_165426395473886 37329& ev_chn=loja& s_kwcid=AL!4428!3 !386247001345!!! g!856907751004!& gad_source=1</td></tr><tr><td>Tubos Flexíveis Microcentrífuga</td><td>Eppendorf&nbsp;</td><td>EP022364120</td><td>Tubos de centrífuga; https://www.sigmaaldrich.com/US/en/product/sigma/ep022364120?utm_source=google%2Cgoogle&utm _medium=organicshopping%2Ccpc& utm _campaign=21043330280& utm_ conteúdo=& gclid=Cj0KCQjwpv K4BhDUARIsADHt9sTHRD35k CHPtfI2A41axodnMVr6a1eBKk zM4bSUYQAyfEKo3UgTAEQa Ap7wEALw_wcB</td></tr><tr><td>Gás Nitrogênio</td><td>Airgas</td><td>N/A</td><td></td></tr><tr><td>Lâminas de microscópio de vidro</td><td>Microscopia Eletrônica Ciências</td><td>71873-02</td><td>https://www.emsdiasum.com/positive-charge-microscope-slides</td></tr><tr><td>Nitrogênio Líquido</td><td>Airgas</td><td>N/A</td><td></td></tr><tr><td>Espectrômetro de Massa</td><td>Waters</td><td>Synapt G2-Si</td><td>https://www.waters.com/waters/en_US/SYNAPT-G2-Si-Mass-Spectrometry/nav.htm?locale=en_mkcid=1000251Foodety%3C/a%3E&cid=134740653&bcid= 134528734</td></tr><tr><td>Pulverizador de Matriz</td><td>HTX Technologies</td><td>M3+</td><td>https://www.htximaging.com/htx-m3-sprayer</td></tr><tr><td>Metanol (HPLC), >99,9%</td><td>Fisher Chemical&nbsp;</td><td>A4524</td><td>Metanol; https://www.fishersci.com/shop/products/methanol-hplc-fisher-chemical-9/A4524?crossRef=A4524#?keyword=A4524</td></tr><tr><td>Sistema de Ultrassom Pré-clínico&nbsp;</td><td>FUJIFILM VisualSonics</td><td>Vevo 3100</td><td>https://www.visualsonics.com/product/imaging-systems/vevo-3100; O Vevo F2 substituiu o Vevo 3100 na produção. O sistema inclui vaporizador de isoflurano e caixa de indução.</td></tr><tr><td>Reynolds Wrap</td><td>N/A</td><td>N/A</td><td>Folha de alumínio</td></tr><tr><td>Signagel&nbsp; Gel de Eletrodo</td><td>Parker Laboratories Parker</td><td>15-60</td><td>Gel Condutor de Eletrodo; https://www.parkerlabs.com/products/signagel-electrode-gel/</td></tr> <tr><td>Geléia Lubrificante Estéril</td><td>Medline&nbsp;</td><td>MDS032273Z</td><td>Gel Lubrificante; https://www.medline.com/ce/product/Sterile-Lubricating-Jelly/Lubricating-Jelly/Z05-PF03664?sku=MDPMDS032273H</td></tr><tr><td>Instrumentos cirúrgicos: tesouras, pinças, suturas</td><td>Ferramentas de Belas Ciências</td><td>11252-00, 11050-10, 14016-14, 14084-08, 15000-08</td><td>info@finescience.com</td></tr><tr><td>Esponjas Cirúrgicas 200 Pack - ndash; Almofadas de Gaze Não Estéreis - Primeiros Socorros Esponja de Curativo para Cuidados com Feridas &ndash; Tecido Médico Antiaderente, Esfregante de Malha Não Aderente</td><td>Medpride&nbsp;</td><td>B08RZGQ5GW</td><td>Gaze; https://www.amazon.com/Medpride-Surgical-Sponges-200-Pack/dp/B08RZGQ5GW/ref=asc_df_B08RZGQ5GW/?tag=hyprod-20&linkCode=df0&h vadid=693270340506& hvpos= & hvnetw=g& hvrand=960915122 2290977669& hvpone=& hvptwo= & hvqmt=& hvdev=c& hvdvcmdl=& hvlocint=& hvlocphy=9192978& hv targid=pla-1245491514869& psc= 1& mcid=33f4d647c88630c79116 888d565a63b0</td></tr><tr><td>Composto Tissue-Plus O.C.T.&nbsp;</td><td>Fisher Scientific</td><td>23-730-571</td><td>OCT; https://www.fishersci.com/shop/products/tissue-plus-o-c-t-compound-2/23730571</td></tr><tr><td>Cotonetes e Aplicadores com Cabo de Madeira</td><td>Fisherbrand&nbsp;</td><td>22-363-160</td><td>Cotonete; https://www.fishersci.com/shop/products/wood-handled-cotton-swabs-applicators-8/p-7146852</td></tr></tbody></table>

estudo multimodal da remodelação cardiovascular murina: ultrassom quadridimensional e imagem por espectrometria de massas

Multimodal Study of Murine Cardiovascular Remodeling: Four-Dimensional Ultrasound and Mass Spectrometry Imaging

Os protocolos de imagem descritos acima foram utilizados para dois estudos preliminares: remodelamento do infarto do miocárdio (IM) e envelhecimento vascular. Para os experimentos cardíacos, foi realizada uma cirurgia de ligadura definitiva da artéria coronária para induzir infarto agudo do miocárdio18,19. O ultrassom 4D e o MALDI MSI foram realizados progressivamente no mesmo tecido, revelando alterações fisiológicas e moleculares. Imagens representativas de íon...

Estudo Multimodal da Remodelação Cardiovascular Murina: Ultrassom Quadridimensional e Imagem por Espectrometria de Massas

Cardiovascular disease (CVD) is the leading cause of death in the United States. Damage in&#160;the cardiovascular system can be due to environmental ...

multimodal-study-murine-cardiovascular-remodeling-four-dimensional

Research

JoVE Journal

Bioengineering

Bioengenharia

Este vídeo demonstra o uso de um trilho montado de alta freqüência da sonda de ultra-som para realizar ecocardiografia de um rato anestesiado. Os métodos de descrever ambas as medições convencionais bidimensional e modo-M da função cardíaca, além de mais novos, ferramentas mais poderosas, como a cor Doppler, análise de tensão, bem como imagens de contraste gerais e específicas.

Ecocardiografia murino e ultra-sonografia

A ecocardiografia é comumente usada para caracterizar e quantificar alterações na estrutura e função cardíaca. Descrevemos um algoritmo de imagem baseado em ultrassom que oferece uma medida de substituto aprimorada da microestrutura do miocárdio e pode ser realizado usando software de análise de imagem de acesso aberto.

Avaliação ultrassônica da microestrutura miocárdio

O objetivo deste protocolo é avaliar de forma não invasiva alterações cardíacas estruturais e funcionais em um rato modelo da doença de coração criado por constrição aórtica transversal, usando B e M-mode ecocardiografia e cor / impulso de imagem onda Doppler.

Avaliação da morfológicas e funcionais cardíacas Mudanças no Modelo rato de Transverse aórtica constrição por ecocardiográfica de imagem

Este protocolo fornece uma descrição detalhada da abordagem ecocardiográfica para fenotipagem abrangente do coração e da função da válvula cardíaca em camundongos.

Abordagens ecocardiograma e protocolos de Comprehensive Caracterização fenotípica de doença cardíaca valvular em ratos

Ultra-sonografia tornou-se uma modalidade comum para determinar as dimensões luminal dos aneurismas da aorta torácicas e abdominais em ratos. Este protocolo descreve o procedimento para adquirir imagens de ultrassom bidimensional confiável e reprodutível da aorta ascendente e abdominal em ratos.

Ecografia da Aorta torácica e Abdominal em ratos para determinar as dimensões de aneurisma

Este estudo descreve um protocolo abrangente de ressonância magnética cardiovascular (RMC) para quantificar os parâmetros funcionais ventriculares esquerdos do coração do camundongo. O protocolo descreve a aquisição, pós-processamento e análise das imagens da RMC, bem como a avaliação de diferentes parâmetros funcionais cardíacos.

Quantificação da função ventricular esquerda do coração do rato, tensão do miocárdio e forças hemodinâmicas por ressonância magnética cardiovascular

Este protocolo demonstra o ultrassom robótico (EUA) como uma alternativa prática, econômica e rápida às modalidades tradicionais de imagem não invasiva.

Uso de ultrassom robótico 3D para análise in vivo de rins de rato

Relatamos um método para reconstrução mesoscópica de todo o coração do rato, combinando novos avanços na transformação e coloração de tecidos com o desenvolvimento de um microscópio de folha de luz digitalizado axialmente.

Imagem óptica mesoscópica do coração de todo o rato

O protocolo utiliza microscopia avançada de folha de luz junto com métodos adaptados de limpeza de tecidos para investigar estruturas cardíacas intrincadas em corações de roedores, com grande potencial para a compreensão da morfogênese e remodelação cardíaca.

Imagem de folha de luz para revelar estrutura cardíaca em corações de roedores

A ultrassonografia Doppler ultrarrápida (UFUS) com alta resolução espacial (100 μm) e sensibilidade representa uma modalidade de imagem não invasiva adequada para obter uma visão qualitativa abrangente da vasculatura hepática. O UFUS também facilita as medições quantitativas da microvasculatura, com o objetivo de melhorar nossa compreensão dos mecanismos das doenças vasculares.

Imagem e quantificação da vasculatura hepática de camundongos usando ultrassom Doppler ultrarrápido

Explore a remodelação cardiovascular murina usando ultrassom 4D e espectrometria de massas. Este protocolo integra imagem funcional e molecular para analisar alterações biomecânicas e biomoleculares em tecidos cardíacos e vasculares.

Estudo Multimodal da Remodelação Cardiovascular Murina: Ultrassom Quadridimensional e Imagem por Espectrometria de Massas

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