Name: Método de amostragem de sangue através da parede para minimizar a interrupção do sono em ambientes clínicos (Vídeo) | JoVE Journal
Uploaded: 2025-06-13T14:00:00.000+00:00
Duration: 6 min 39 s
Description: Aprenda um método minimamente invasivo de amostragem sanguínea através da parede para estudar ritmos circadianos e saúde cardiometabólica sem interromper o sono do participante.

ai_agent_IP

ai_agent

maintenance

instantTrialDurationMinutes

isBiopharma

enableNewMyJoveSectionDesign

educationFeatures

seoMetaApi

ai_copilot_institution_blacklist

ai_chat_institution_blacklist

freeAccessWindow

ssoRoleCapture

scheduledMaintenanceBanner

joveLabsFeatures

disable_autoplay_for_bots

extended_trial_duration

5 minutes

<ol><li>Cox, K. H., Takahashi, J. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Circadian+clock+genes+and+the+transcriptional+architecture+of+the+clock+mechanism%5BTitle%5D)+AND+%22J+Mol+Endocrinol%22%5BJournal%5D)">Circadian clock genes and the transcriptional architecture of the clock mechanism.</a> J Mol Endocrinol. 63 (4), R93-R102 (2019).</li>
<li>Broussard, J. L., et al. <a target="_blank" href="http://scholar.google.com/scholar?hl=en&safe=off&q=author%3aBroussard%2C+JL+%22Biological+Timekeeping%3A+Clocks%2C+Rhythms+and+Behaviour%22">Biological Timekeeping: Clocks, Rhythms and Behaviour</a>. , Springer. India. (2017).</li>
<li>Chellappa, S. L., Vujovic, N., Williams, J. S., Scheer, F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Impact+of+circadian+disruption+on+cardiovascular+function+and+disease%5BTitle%5D)+AND+%22Trends+Endocrinol+Metab%22%5BJournal%5D)">Impact of circadian disruption on cardiovascular function and disease.</a> Trends Endocrinol Metab. 30 (10), 767-779 (2019).</li>
<li>Broussard, J. L., Van Cauter, E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Disturbances+of+sleep+and+circadian+rhythms%3A+novel+risk+factors+for+obesity%5BTitle%5D)+AND+%22Curr+Opin+Endocrinol+Diabetes+Obes%22%5BJournal%5D)">Disturbances of sleep and circadian rhythms: novel risk factors for obesity.</a> Curr Opin Endocrinol Diabetes Obes. 23 (5), 353-359 (2016).</li>
<li>Hanlon, E., Van Cauter, E. V., Tasali, E., Broussard, J. <a target="_blank" href="http://scholar.google.com/scholar?hl=en&safe=off&q=author%3aHanlon%2C+E+%22Endocrine+Physiology+in+Relation+to+Sleep+and+Sleep+Disturbances.+Principles+and+Pract+Sleep+Medicine%22">Endocrine Physiology in Relation to Sleep and Sleep Disturbances. Principles and Pract Sleep Medicine</a>. , Elsevier Saunders, MO. (2021).</li>
<li>Prasad, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((A+smartphone+intervention+to+promote+time+restricted+eating+reduces+body+weight+and+blood+pressure+in+adults+with+overweight+and+obesity%3A+a+pilot+study%5BTitle%5D)+AND+%22Nutrients%22%5BJournal%5D)">A smartphone intervention to promote time restricted eating reduces body weight and blood pressure in adults with overweight and obesity: a pilot study.</a> Nutrients. 13 (7), 2148(2021).</li>
<li>Procopio, S. B., Esser, K. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Clockwork+conditioning%3A+aligning+the+skeletal+muscle+clock+with+time-of-day+exercise+for+cardiometabolic+health%5BTitle%5D)+AND+%22J+Mol+Cell+Cardiol%22%5BJournal%5D)">Clockwork conditioning: aligning the skeletal muscle clock with time-of-day exercise for cardiometabolic health.</a> J Mol Cell Cardiol. 198, 36-44 (2025).</li>
<li>Holtman, S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Changes+in+circadian+timing+following+a+one-week+in-home+sleep+extension+manipulation+in+habitually+short-sleeping+adolescents%5BTitle%5D)+AND+%22Sleep%22%5BJournal%5D)">Changes in circadian timing following a one-week in-home sleep extension manipulation in habitually short-sleeping adolescents.</a> Sleep. 48 (2), zsae273(2024).</li>
<li>Chellappa, S. L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Daytime+eating+prevents+internal+circadian+misalignment+and+glucose+intolerance+in+night+work%5BTitle%5D)+AND+%22Sci+Adv%22%5BJournal%5D)">Daytime eating prevents internal circadian misalignment and glucose intolerance in night work.</a> Sci Adv. 7, eabg9910(2021).</li>
<li>Skene, D. J., Arendt, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Human+circadian+rhythms%3A+physiological+and+therapeutic+relevance+of+light+and+melatonin%5BTitle%5D)+AND+%22Ann+Clin+Biochem%22%5BJournal%5D)">Human circadian rhythms: physiological and therapeutic relevance of light and melatonin.</a> Ann Clin Biochem. 43 (Pt 5), 344-353 (2006).</li>
<li>Powell, D. J., Schlotz, W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Daily+life+stress+and+the+cortisol+awakening+response%3A+testing+the+anticipation+hypothesis%5BTitle%5D)+AND+%22PLoS+One%22%5BJournal%5D)">Daily life stress and the cortisol awakening response: testing the anticipation hypothesis.</a> PLoS One. 7 (12), e52067(2012).</li>
<li>Wright, K. P., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Influence+of+sleep+deprivation+and+circadian+misalignment+on+cortisol%2C+inflammatory+markers%2C+and+cytokine+balance%5BTitle%5D)+AND+%22Brain+Behav+Immun%22%5BJournal%5D)">Influence of sleep deprivation and circadian misalignment on cortisol, inflammatory markers, and cytokine balance.</a> Brain Behav Immun. 47, 24-34 (2015).</li>
<li>Ikegami, K., Refetoff, S., Van Cauter, E., Yoshimura, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Interconnection+between+circadian+clocks+and+thyroid+function%5BTitle%5D)+AND+%22Nat+Rev+Endocrinol%22%5BJournal%5D)">Interconnection between circadian clocks and thyroid function.</a> Nat Rev Endocrinol. 15 (10), 590-600 (2019).</li>
<li>Kessler, L., Nedeltcheva, A., Imperial, J., Penev, P. D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Changes+in+serum+TSH+and+free+T4+during+human+sleep+restriction%5BTitle%5D)+AND+%22Sleep%22%5BJournal%5D)">Changes in serum TSH and free T4 during human sleep restriction.</a> Sleep. 33 (8), 1115-1118 (2010).</li>
<li>Van Cauter, E., Plat, L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Physiology+of+growth+hormone+secretion+during+sleep%5BTitle%5D)+AND+%22J+Pediatr%22%5BJournal%5D)">Physiology of growth hormone secretion during sleep.</a> J Pediatr. 128 (5 Pt 2), S32-S37 (1996).</li>
<li>Morselli, L., Leproult, R., Balbo, M., Spiegel, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Role+of+sleep+duration+in+the+regulation+of+glucose+metabolism+and+appetite%5BTitle%5D)+AND+%22Best+Pract+Res+Clin+Endocrinol+Metab%22%5BJournal%5D)">Role of sleep duration in the regulation of glucose metabolism and appetite.</a> Best Pract Res Clin Endocrinol Metab. 24 (5), 687-702 (2010).</li>
<li>Kapcala, L. P., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Twenty-four-hour+prolactin+secretory+patterns+in+women+with+galactorrhea%2C+normal+menses%2C+normal+random+prolactin+levels+and+abnormal+sellar+tomograms%5BTitle%5D)+AND+%22J+Endocrinol+Invest%22%5BJournal%5D)">Twenty-four-hour prolactin secretory patterns in women with galactorrhea, normal menses, normal random prolactin levels and abnormal sellar tomograms.</a> J Endocrinol Invest. 7 (5), 455-460 (1984).</li>
<li>Sassin, J. F., Frantz, A. G., Kapen, S., Weitzman, E. D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((The+nocturnal+rise+of+human+prolactin+is+dependent+on+sleep%5BTitle%5D)+AND+%22J+Clin+Endocrinol+Metab%22%5BJournal%5D)">The nocturnal rise of human prolactin is dependent on sleep.</a> J Clin Endocrinol Metab. 37 (3), 436-440 (1973).</li>
<li>Chaput, J. -P., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((The+role+of+insufficient+sleep+and+circadian+misalignment+in+obesity%5BTitle%5D)+AND+%22Nat+Rev+Endocrinol%22%5BJournal%5D)">The role of insufficient sleep and circadian misalignment in obesity.</a> Nat Rev Endocrinol. 19 (2), 82-97 (2023).</li>
<li>Broussard, J. L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Sleep+restriction+increases+free+fatty+acids+in+healthy+men%5BTitle%5D)+AND+%22Diabetologia%22%5BJournal%5D)">Sleep restriction increases free fatty acids in healthy men.</a> Diabetologia. 58 (4), 791-798 (2015).</li>
<li>Vaughn, L. K., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Endocannabinoid+signalling%3A+has+it+got+rhythm%5BTitle%5D)+AND+%22Br+J+Pharmacol%22%5BJournal%5D)">Endocannabinoid signalling: has it got rhythm.</a> Br J Pharmacol. 160 (3), 530-543 (2010).</li>
<li>Lindgren, O., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Differential+islet+and+incretin+hormone+responses+in+morning+versus+afternoon+after+standardized+meal+in+healthy+men%5BTitle%5D)+AND+%22J+Clin+Endocrinol+Metab%22%5BJournal%5D)">Differential islet and incretin hormone responses in morning versus afternoon after standardized meal in healthy men.</a> J Clin Endocrinol Metab. 94 (8), 2887-2892 (2009).</li>
<li>Wesselius, H. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Quality+and+quantity+of+sleep+and+factors+associated+with+sleep+disturbance+in+hospitalized+patients%5BTitle%5D)+AND+%22JAMA+Intern+Med%22%5BJournal%5D)">Quality and quantity of sleep and factors associated with sleep disturbance in hospitalized patients.</a> JAMA Intern Med. 178 (9), 1201-1208 (2018).</li>
<li>Markwald, R. R., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Impact+of+insufficient+sleep+on+total+daily+energy+expenditure%2C+food+intake%2C+and+weight+gain%5BTitle%5D)+AND+%22Proc+Natl+Acad+Sci+U+S+A%22%5BJournal%5D)">Impact of insufficient sleep on total daily energy expenditure, food intake, and weight gain.</a> Proc Natl Acad Sci U S A. 110 (14), 5695-5700 (2013).</li>
<li>McHill, A. W., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Impact+of+circadian+misalignment+on+energy+metabolism+during+simulated+nightshift+work%5BTitle%5D)+AND+%22Proc+Natl+Acad+Sci+U+S+A%22%5BJournal%5D)">Impact of circadian misalignment on energy metabolism during simulated nightshift work.</a> Proc Natl Acad Sci U S A. 111 (48), 17302-17307 (2014).</li>
<li>Depner, C. M., Melanson, E. L., McHill, A. W., Wright, K. P. Jr <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Mistimed+food+intake+and+sleep+alters+24-hour+time-of-day+patterns+of+the+human+plasma+proteome%5BTitle%5D)+AND+%22Proc+Natl+Acad+Sci+U+S+A%22%5BJournal%5D)">Mistimed food intake and sleep alters 24-hour time-of-day patterns of the human plasma proteome.</a> Proc Natl Acad Sci U S A. 115 (23), E5390-E5399 (2018).</li>
<li>Mullington, J. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Sleep+loss+reduces+diurnal+rhythm+amplitude+of+leptin+in+healthy+men%5BTitle%5D)+AND+%22J+Neuroendocrinol%22%5BJournal%5D)">Sleep loss reduces diurnal rhythm amplitude of leptin in healthy men.</a> J Neuroendocrinol. 15 (9), 851-854 (2003).</li>
<li>Ona, E., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((A+technique+for+improved+blood+sampling+during+sleep+studies%5BTitle%5D)+AND+%22Psychophysiology%22%5BJournal%5D)">A technique for improved blood sampling during sleep studies.</a> Psychophysiology. 31 (6), 609-610 (1994).</li>
<li>Schlierf, G., Dorow, E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Diurnal+patterns+of+triglycerides%2C+free+fatty+acids%2C+blood+sugar%2C+and+insulin+during+carbohydrate-induction+in+man+and+their+modification+by+nocturnal+suppression+of+lipolysis%5BTitle%5D)+AND+%22J+Clin+Invest%22%5BJournal%5D)">Diurnal patterns of triglycerides, free fatty acids, blood sugar, and insulin during carbohydrate-induction in man and their modification by nocturnal suppression of lipolysis.</a> J Clin Invest. 52 (3), 732-740 (1973).</li>
<li>Schlierf, G., Reinheimer, W., Stossberg, V. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Diurnal+patterns+of+plasma+triglycerides+and+free+fatty+acids+in+normal+subjects+and+in+patients+with+endogenous+%28type+IV%29+hyperlipoproteinemia%5BTitle%5D)+AND+%22Nutr+Metab%22%5BJournal%5D)">Diurnal patterns of plasma triglycerides and free fatty acids in normal subjects and in patients with endogenous (type IV) hyperlipoproteinemia.</a> Nutr Metab. 13 (2), 80-91 (1971).</li>
<li>Swanson, C. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((turnover+markers+after+sleep+restriction+and+circadian+disruption%3A+a+mechanism+for+sleep-related+bone+loss+in+humans%5BTitle%5D)+AND+%22J+Clin+Endocrinol+Metab%22%5BJournal%5D)">turnover markers after sleep restriction and circadian disruption: a mechanism for sleep-related bone loss in humans.</a> J Clin Endocrinol Metab. 102 (10), 3722-3730 (2017).</li>
<li>Van Cauter, E., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Modulation+of+glucose+regulation+and+insulin+secretion+by+circadian+rhythmicity+and+sleep%5BTitle%5D)+AND+%22J+Clin+Invest%22%5BJournal%5D)">Modulation of glucose regulation and insulin secretion by circadian rhythmicity and sleep.</a> J Clin Invest. 88 (3), 934-942 (1991).</li>
<li>Rynders, C. A., Morton, S. J., Bessesen, D. H., Wright, K. P. Jr, Broussard, J. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Circadian+rhythm+of+substrate+oxidation+and+hormonal+regulators+of+energy+balance%5BTitle%5D)+AND+%22Obesity+%28Silver+Spring%29%22%5BJournal%5D)">Circadian rhythm of substrate oxidation and hormonal regulators of energy balance.</a> Obesity (Silver Spring). 28 (Suppl 1), S104-S113 (2020).</li>
<li>Thosar, S. S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Circadian+rhythm+of+vascular+function+in+midlife+adults%5BTitle%5D)+AND+%22Arterioscler+Thromb+Vasc+Biol%22%5BJournal%5D)">Circadian rhythm of vascular function in midlife adults.</a> Arterioscler Thromb Vasc Biol. 39 (6), 1203-1211 (2019).</li>
<li>Thosar, S. S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Morning+impairment+in+vascular+function+is+unrelated+to+overnight+sleep+or+the+inactivity+that+accompanies+sleep%5BTitle%5D)+AND+%22Am+J+Physiol+Regul+Integr+Comp+Physiol%22%5BJournal%5D)">Morning impairment in vascular function is unrelated to overnight sleep or the inactivity that accompanies sleep.</a> Am J Physiol Regul Integr Comp Physiol. 315 (5), R986-R993 (2018).</li>
<li>Dimsdale, J. E., Coy, T., Ziegler, M. G., Ancoli-Israel, S., Clausen, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((The+effect+of+sleep+apnea+on+plasma+and+urinary+catecholamines%5BTitle%5D)+AND+%22Sleep%22%5BJournal%5D)">The effect of sleep apnea on plasma and urinary catecholamines.</a> Sleep. 18 (5), 377-381 (1995).</li>
<li>Klerman, E. B., Gershengorn, H. B., Duffy, J. F., Kronauer, R. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Comparisons+of+the+variability+of+three+markers+of+the+human+circadian+pacemaker%5BTitle%5D)+AND+%22J+Biol+Rhythms%22%5BJournal%5D)">Comparisons of the variability of three markers of the human circadian pacemaker.</a> J Biol Rhythms. 17 (2), 181-193 (2002).</li>
<li>Benloucif, S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Measuring+melatonin+in+humans%5BTitle%5D)+AND+%22J+Clin+Sleep+Med%22%5BJournal%5D)">Measuring melatonin in humans.</a> J Clin Sleep Med. 4 (1), 66-69 (2008).</li>
<li>Lammers, W. J., Badia, P., Hughes, R., Harsh, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Temperature%2C+time-of-night+of+testing%2C+and+responsiveness+to+stimuli+presented+while+sleeping%5BTitle%5D)+AND+%22Psychophysiology%22%5BJournal%5D)">Temperature, time-of-night of testing, and responsiveness to stimuli presented while sleeping.</a> Psychophysiology. 28 (4), 463-467 (1991).</li>
<li>Alikhan, R., Forster, R., Cohen, A. T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Heparin+for+the+prevention+of+venous+thromboembolism+in+acutely+ill+medical+patients+%28excluding+stroke+and+myocardial+infarction%29%5BTitle%5D)+AND+%22Cochrane+Database+Syst+Rev%22%5BJournal%5D)">Heparin for the prevention of venous thromboembolism in acutely ill medical patients (excluding stroke and myocardial infarction).</a> Cochrane Database Syst Rev. 2014 (5), CD003747(2014).</li>
<li>Roth, B., Ekelund, M., Fan, B. G., Ekstrom, U., Nilsson-Ehle, P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Effects+of+heparin+and+low+molecular+weight+heparin+on+lipid+transport+during+parenteral+feeding+in+the+rat%5BTitle%5D)+AND+%22Acta+Anaesthesiol+Scand%22%5BJournal%5D)">Effects of heparin and low molecular weight heparin on lipid transport during parenteral feeding in the rat.</a> Acta Anaesthesiol Scand. 40 (1), 102-111 (1996).</li>
<li>Weintraub, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Continuous+intravenous+heparin+administration+in+humans+causes+a+decrease+in+serum+lipolytic+activity+and+accumulation+of+chylomicrons+in+circulation%5BTitle%5D)+AND+%22J+Lipid+Res%22%5BJournal%5D)">Continuous intravenous heparin administration in humans causes a decrease in serum lipolytic activity and accumulation of chylomicrons in circulation.</a> J Lipid Res. 35 (2), 229-238 (1994).</li>
<li>Schallom, M. E., Prentice, D., Sona, C., Micek, S. T., Skrupky, L. P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Heparin+or+0.9%25+sodium+chloride+to+maintain+central+venous+catheter+patency%3A+a+randomized+trial%5BTitle%5D)+AND+%22Crit+Care+Med%22%5BJournal%5D)">Heparin or 0.9% sodium chloride to maintain central venous catheter patency: a randomized trial.</a> Crit Care Med. 40 (6), 1820-1826 (2012).</li>
<li>Rahman, S. A., Wright, K. P. Jr, Lockley, S. W., Czeisler, C. A., Gronfier, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Characterizing+the+temporal+dynamics+of+melatonin+and+cortisol+changes+in+response+to+nocturnal+light+exposure%5BTitle%5D)+AND+%22Sci+Rep%22%5BJournal%5D)">Characterizing the temporal dynamics of melatonin and cortisol changes in response to nocturnal light exposure.</a> Sci Rep. 9 (1), 19720(2019).</li>
</ol>

<table><tbody><tr><td>Conjunto de extensão IV de diâmetro padrão de 9 polegadas com volume de priming de 1 mL, conector SmartSite removível sem agulha, grampo deslizante e trava de luer macho de rotação</td><td>Medline</td><td>IME22059EH</td><td></td></tr><tr><td>Injeção de cloreto de sódio a 0,9% USP 10 mL em uma seringa ZR de 10 mL de lavagem</td><td>Excelsior Medical</td><td>EMZE010001</td><td></td></tr><tr><td>Absorvente de preparação para álcool</td><td>Medline</td><td>MDS090737</td><td></td></tr><tr><td>Cateter alado BD Insyte Autoguard de 20 G</td><td>Medline</td><td>381534</td><td></td></tr><tr><td>Seringa BD Plastipak 3 mL</td><td>Fischer Scientific</td><td>309657</td><td></td></tr><tr><td>CoFlexNL</td><td>Medline</td><td>5200CP</td><td></td></tr><tr><td>Conjunto de extensão IV de 192 pol, tubulação de pequeno diâmetro</td><td>Medicina da UTI</td><td>B2202</td><td>É necessário criar uma conta na UTI médica para solicitar suprimentos</td></tr><tr><td>Esponjas de gaze não tecidas</td><td>Medline</td><td>NON25223</td><td></td></tr><tr><td>Tegaderm</td><td>Medline</td><td>1624W</td><td></td></tr><tr><td>Ultra(TM) trava de 3 posições para stockcockw/giratória macho luer</td><td>Medline</td><td>MX2311L</td><td></td></tr></tbody></table>

método de amostragem de sangue através da parede para minimizar a interrupção do sono em ambientes clínicos

Through-the-Wall Blood Sampling Method to Minimize Sleep Disruption in Clinical Settings

Método de amostragem de sangue através da parede para minimizar a interrupção do sono em ambientes clínicos

Physiological rhythms, including diurnal and circadian rhythms, play a critical role in health, with disturbances increasingly linked to cardiovascular ...

through-wall-blood-sampling-method-to-minimize-sleep-disruption

Research

JoVE Journal

Medicine

Medicina

Cateterização crônica dos vasos sangüíneos no rato é muitas vezes necessária para a administração de substâncias, obter amostra de sangue durante um período de tempo ou para medições de pressão direta consciente sangue. Cateterismo arterial femoral do rato e medidas correspondentes da pressão arterial em que o animal consciente será demonstrado.

Cateterismo arterial e venoso femoral para coleta de sangue, Drug Administration e Pressão Arterial Consciente e Medidas de freqüência cardíaca

Descrevemos uma metodologia para o processamento de sangue total para obter uma variedade de componentes para análise posterior. Otimizamos um protocolo simplificado que permite o processamento simultâneo rápido e de alto rendimento de amostras de sangue total em um ambiente não clínico.

Fracionamento rápido e isolamento de componentes de sangue total em amostras obtidas a partir de uma configuração de base comunitária

Aqui, apresentamos um protocolo para melhorar a qualidade dos dados de sono em casa testando fornecendo um método para melhorar instruções através de uma visita de participante estruturada. Este protocolo inclui a implementação de um manual de ensino passo a passo com fotos para assegurar a colocação adequada do equipamento.

Multi-Modal casa sono monitoramento em adultos mais velhos

A perda de sono e o desalinhamento circadiano contribuem para inúmeros acidentes e incidentes operacionais. A efetividade das contramedidas e dos projetos de agendamento de trabalho visando mitigar a fadiga pode ser um desafio para avaliar em ambientes operacionais. Este manuscrito resume uma abordagem para coleta de dados de sono, circadiano, fadiga e desempenho em ambientes operacionais complexos.

Coletando dados de sono, circadiano, fadiga e desempenho em ambientes operacionais complexos

Aqui, apresentamos um método para investigar ritmos diurnos em desempenho após categorização precisa dos participantes em grupos fenótipos circadianos com base no Questionário cronotipo de Munique, biomarcadores de fase circadiana padrão ouro e medidas actigráficas.

Fenotipagem circadiana humana e testes de desempenho diurno no mundo real

O presente protocolo descreve um método simples e eficiente de coleta de sangue da veia subclávia em ratos. Permite uma amostragem rápida, oportuna e facilmente identificável sem anestesia e obtém sangue de alta qualidade através da coleta repetitiva de amostras.

Amostragem Repetitiva de Sangue da Veia Subclávia de Rato Consciente

O presente protocolo descreve um método simples e eficiente para a coleta dinâmica e em tempo real de sangue de coração de ratos utilizando a técnica de microdiálise.

Extração Contínua Dinâmica de Sangue do Coração de Rato via Técnica de Microdiálise Não Invasiva

O protocolo mostra repetidas coletas de líquido cefalorraquidiano e sangue de ratos epilépticos realizadas em paralelo com a monitorização contínua por vídeo-eletroencefalograma (EEG). Estes são instrumentais para explorar possíveis ligações entre alterações em várias moléculas de fluido corporal e atividade convulsiva.

Amostragem de líquido cefalorraquidiano e sangue da veia lateral da cauda em ratos durante registros de EEG

A pulsatilidade do hormônio luteinizante (LH) é uma característica da função reprodutiva. Descrevemos um protocolo para ativação remota de populações neuronais específicas ligado à coleta de sangue automatizada seriada. Esta técnica permite modulação hormonal cronometrada, multiplexação e minimização dos efeitos de manipulação sobre os níveis de LH em animais conscientes, em movimento livre e não perturbados.

Ativação Neuronal Remota Acoplada à Coleta Automatizada de Sangue para Induzir e Medir o Hormônio Luteinizante Circulante em Camundongos

Apresentamos aqui uma combinação de métodos eficazes de restrição em ratos e punção da veia subclávia que permitem a coleta de sangue rápida, segura e repetida em ratos sem anestesia.

Coleta de sangue da veia subclávia em ratos conscientes

Aprenda um método minimamente invasivo de amostragem sanguínea através da parede para estudar ritmos circadianos e saúde cardiometabólica sem interromper o sono do participante.

Método de amostragem de sangue através da parede para minimizar a interrupção do sono em ambientes clínicos

Explorar mais vídeos