Name: Modelo de ingestão periódica intermitente em camundongos (Vídeo) | JoVE Journal
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Description: Aprenda a estabelecer um modelo de ingestão periódica intermitente em camundongos usando alimentos palatáveis. Este método permite estudar comportamentos de compulsão alimentar e ansiedade em transtornos alimentares.

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<meta charset="utf8"></head><body>Investigando o transtorno da compulsão alimentar periódica com um modelo de camundongo de acesso a alimentos palatáveis

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<li>Awad, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Altered+reward+processing+following+sucrose+bingeing+in+male+and+female+mice%5BTitle%5D)+AND+%22Nutr+Neurosci%22%5BJournal%5D)">Altered reward processing following sucrose bingeing in male and female mice.</a> Nutr Neurosci. 27 (11), 1269-1282 (2024).</li>
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</ol>

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modelo de ingestão periódica intermitente em camundongos

Intermittent Binge-Intake Model in Mice

Usando HPF, estabelecemos com sucesso um modelo de compulsão alimentar em camundongos C57BL / 6. No dia 8, o comportamento de compulsão alimentar (consumir alimentos em excesso por 2 h) foi firmemente estabelecido e mantido até o dia 26 (Figura 1A). Durante as sessões intermitentes, o grupo GI exibiu consumo total de kcal significativamente maior (tanto da ração padrão quanto do HPF do que os grupos GC e CoG) (Figura 1A, ### p &lt; 0,0001, *** p &lt; 0,0001, ** ...

Modelo de ingestão periódica intermitente em camundongos

Obesity affects one in eight individuals globally. Overeating, especially high-calorie foods, plays a significant role in obesity. Binge eating disorder ...

Research

JoVE Journal

Behavior

Comportamento

Aqui apresentamos um protocolo para induzir anorexia baseada em atividade (ABA) em camundongos adolescentes fêmeas. ABA é uma condição de hiperatividade evocada pela imposição de restrição alimentar a roedores com acesso a uma roda de corrida. Esse fenômeno está sendo usado como modelo para estudar a neurobiologia subjacente da anorexia nervosa.

Usando a Anorexia Rodent modelo baseado em atividades para estudar a base neurobiológica da Anorexia Nervosa

Transtorno de uso de álcool (AUD) é um problema de saúde nacional e o desenvolvimento de tratamentos mais eficazes é necessário para compensar as necessidades desta população de pacientes. Para este fim, o seguinte protocolo utiliza dois modelos simples roedores bebendo para avaliar a eficácia pré-clínica de compostos de chumbo anti-álcool.

Murino bebendo modelos no desenvolvimento de farmacoterapias para alcoolismo: beber na escolha escura e dois-garrafa

Ratinhos alojados individualmente com uma roda de execução enquanto dado acesso limitado ao alimento desenvolvem reduções no consumo de alimentos e aumentam a atividade na roda de execução. Este fenômeno experimental é chamado anorexia baseado na atividade. Este paradigma fornece uma ferramenta experimental para estudar a neurobiologia e comportamentos subjacentes aspectos da anorexia nervosa.

Avaliar a Anorexia baseado na atividade em ratos

O artigo atual descreve um protocolo detalhado para o jejum intermitente isocalístico 2:1 para proteger e tratar de encontro à obesidade e ao metabolismo danificado da glicose em ratos do selvagem-tipo e do ob/ob.

Avaliação dos efeitos metabólicos do jejum intermitente isocalórico 2:1 em camundongos

A restrição do tempo de ingestão alimentar tem emergido como uma intervenção promissora para atenuar doenças metabólicas induzidas pela dieta. Este manuscrito detalha a construção e o uso de um eficiente sistema construído internamente para medir e manipular a ingestão rítmica de alimentos em camundongos.

Manipulação da Ingestão Rítmica de Alimentos em Camundongos Utilizando um Sistema de Alimentação Customizado

Apresentado aqui está um protocolo para o modelo 2BC/CIE de dependência de álcool em camundongos para estudar o transtorno por uso de álcool.

Exposição Crônica Intermitente ao Vapor de Etanol Emparelhada com Escolha de Duas Garrafas para Modelar o Transtorno por Uso de Álcool

Este protocolo descreve um paradigma padrão de consumo doméstico de duas garrafas de acesso intermitente para modelar o consumo de álcool em ratos. Além disso, fornece instruções passo a passo para aumentar o protocolo padrão com um sistema de detecção de lambedura DIY que permite a análise microestrutural do comportamento de beber.

Modelagem do consumo de álcool em roedores usando o consumo de álcool em gaiola doméstica de escolha de duas garrafas e análise microestrutural

No experimento seguinte, descrevemos um protocolo para traço medo condicionado em ratos. Este tipo de memória associativa inclui um período de traço que separa o estímulo neutro eo estímulo incondicionado.

Traçar medo condicionado em ratos

Este protocolo descreve a placa de furo modificada, que é uma configuração de teste comportamental que compreende as características de um campo aberto e uma placa de furo tradicional. Essa configuração permite a análise diferencial do comportamento incondicionado de pequenos mamíferos de laboratório, bem como a análise das habilidades cognitivas.

The Hole Board Modificado - Medir Comportamento, Cognição e Interação Social em camundongos e ratos

Este manuscrito descreve um protocolo para examinar o sistema olfativo de roedores. O teste de habituação/deshabituação olfativa permitirá que os investigadores determinem se um camundongo se habitua a um odor apresentado repetidamente e se o camundongo demonstra deshabituação quando apresentado a um novo odor.

Testes para Odor Discriminação e habituação em ratos

Aprenda a estabelecer um modelo de ingestão periódica intermitente em camundongos usando alimentos palatáveis. Este método permite estudar comportamentos de compulsão alimentar e ansiedade em transtornos alimentares.

Modelo de ingestão periódica intermitente em camundongos

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