Name: Identificação de micróglia e macrófagos infiltrantes periféricos na medula espinhal lesada usando citometria de fluxo (Vídeo) | JoVE Journal
Uploaded: 2025-06-24T13:40:00.000+00:00
Duration: 8 min 47 s
Description: Aprenda a identificar micróglia e macrófagos infiltrantes na medula espinhal lesada usando citometria de fluxo otimizada. Este método permite distinguir fenótipos M1 e M2 para estudar neuroinflamação.

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</ol>

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identificação de micróglia e macrófagos infiltrantes periféricos na medula espinhal lesada usando citometria de fluxo

Identifying Microglia and Peripheral Infiltrating Macrophages in the Injured Spinal Cords Using Flow Cytometry

As células imunes do tecido da medula espinhal são separadas usando sílica coloidal revestida com centrifugação por gradiente de densidade de polivinilpirrolidona. Durante o processo de centrifugação, as células são distribuídas em diferentes camadas de acordo com suas densidades flutuantes, conseguindo assim a separação celular (Figura 1). De cima para baixo, a distribuição é a seguinte: detritos celulares, camada rica em células mononucleares, camada rica em linfócitos e, na parte inferior...

Identificação de micróglia e macrófagos infiltrantes periféricos na medula espinhal lesada usando citometria de fluxo

In a healthy spinal cord, peripheral infiltrating macrophages are almost undetectable, and microglia (MG) participate in maintaining the stability of the ...

identifying-microglia-peripheral-infiltrating-macrophages-injured

Research

JoVE Journal

Neuroscience

Neurociência

O presente artigo descreve um protocolo rápido de forma eficiente isolamento de células mononucleares a partir de tecidos do cérebro e da medula espinhal que pode ser efetivamente utilizado para análises de citometria de fluxo.

Isolamento do Cérebro e da Medula Espinhal Células Mononucleares Usando Percoll Gradients

Quantificação da inflamação celular no SNC feridos / patológica por citometria de fluxo é complicada por lipídicas / mielina detritos que podem ter tamanho similar e granulação para as células, diminuindo a sensibilidade / precisão. Temos avançado um método de preparação de células para remover restos de mielina e melhorar a detecção de células por citometria de fluxo na medula lesionada.

Avaliação quantitativa das células imunes no tecido medula lesionada por Citometria de Fluxo: um novo uso para um método de purificação celular

Microglia residem macrófagos que fornecem a primeira linha de defesa e vigilância imune do sistema nervoso central. MicroRNAs são moléculas reguladoras, que desempenham um papel importante em muitos processos fisiológicos, incluindo a activação e diferenciação dos macrófagos. Neste artigo, descrevemos o método para a medição de microRNAs em microglia.

Detecção de MicroRNAs em Microglia por PCR em tempo real no SNC normal e durante Neuroinflammation

Cérebro células mielóides caracterização de acidente vascular cerebral pode ser realizada por estereologia usando o método de fraccionamento da óptica, ou por uma análise de citometria de fluxo de suspensões cérebro leucócitos. Ambos são técnicas úteis para realizar uma distinção fenotípica acurada dos principais subpopulações de células mielóides encontrados no cérebro isquêmico.

Estereológica e Citometria de Fluxo Caracterização de Leucócitos subpopulações em modelos de transitórias ou permanentes Cerebral Isquemia

A imagem intravital de dois fótons pode ser usada para investigar interações entre diferentes tipos de células na medula espinhal em seu ambiente de tecido nativo em um animal quimérico da medula óssea com uma lesão traumática por esmagamento da medula espinhal da coluna dorsal.

Imagem Intravital de axonais Interações com Microglia e macrófagos em um mouse Dorsal Coluna lesão por esmagamento

Este protocolo fornece uma análise das subpopulações de macrófagos no sistema nervoso central do mouse adulto por citometria de fluxo e é útil para o estudo de marcadores múltiplos expressos por essas células.

Análise de Microglia e Macropagos derivados de Monócitos do Sistema Nervoso Central por Citometria de Fluxo

Análise quantitativa do conteúdo da célula dentro do nervo ciático murino é difícil devido à escassez do tecido. Este protocolo descreve um método para a digestão do tecido e preparação que fornece células suficientes para análise de citometria de fluxo de populações de células imunes dos nervos de ratos individuais.

Análise de células do sistema imunológico em único nervos ciático e gânglio da raiz Dorsal de um único rato usando citometria de fluxo

Aqui, apresentamos um protocolo para isolar e purificar a microglia primária em modelos animais de doenças desmielinizantes, utilizando a triagem celular ativada por colunas magnéticas.

Isolamento da Microglia Primária do Rato por Triagem celular ativada por magnético em modelos animais de desmielinização

Aqui, apresentamos um protocolo para avaliar a função das células imunes após AVC isquêmico usando análise de citometria de fluxo em um modelo de mini-AVC. Este método permite o exame detalhado das interações neuroimunes e pode ser adaptado para estudar outras doenças neurodegenerativas, aprimorando nossa compreensão das respostas imunes na patologia do AVC.

Isolamento e Avaliação por Citometria de Fluxo de Interações Neuroimunes em Modelo Murino Mini-AVC

Este protocolo combina citometria de fluxo e sequenciamento de RNA de célula única para isolar e caracterizar os estados das células microgliais no cerebelo de cérebros de camundongos pós-natais precoces. Ele usa dissociação enzimática, centrifugação de Percoll e imunocoloração para revelar a heterogeneidade da microglia e melhorar a compreensão de seus papéis no desenvolvimento e doença cerebelar.

Citometria de fluxo e análise de célula única para caracterizar a ativação da micróglia no desenvolvimento cerebral de camundongos pós-natais precoces

Aprenda a identificar micróglia e macrófagos infiltrantes na medula espinhal lesada usando citometria de fluxo otimizada. Este método permite distinguir fenótipos M1 e M2 para estudar neuroinflamação.

Identificação de micróglia e macrófagos infiltrantes periféricos na medula espinhal lesada usando citometria de fluxo

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