Ocular Therapeutic Delivery and Advanced Tissue Retrieval in Adult Rats

Annie  K. Ryan; Tyler Heisler-Taylor; Stephanie M. Small; Zeina A. Elshaer; Ethan J. Jackson; Vidhya Kannan; Stacey Meeker; Colleen M. Cebulla; Matthew A. Reilly

doi:10.3791/68049

Entrega terapêutica ocular e recuperação avançada de tecidos em ratos adultos

JoVE Journal
Neuroscience

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Neuroscience

Ocular Therapeutic Delivery and Advanced Tissue Retrieval in Adult Rats

Entrega terapêutica ocular e recuperação avançada de tecidos em ratos adultos

Full Text

1,097 Views

06:30 min

May 23, 2025

DOI: 10.3791/68049-v

Annie K. Ryan¹, Tyler Heisler-Taylor², Stephanie M. Small³, Zeina A. Elshaer⁴, Ethan J. Jackson¹, Vidhya Kannan⁵, Stacey Meeker⁶, Colleen M. Cebulla², Matthew A. Reilly^1,2

¹Department of Biomedical Engineering,The Ohio State University, ²Department of Ophthalmology & Visual Sciences,The Ohio State University, ³Department of Mechanical and Aerospace Engineering,The Ohio State University, ⁴Department of Food, Agricultural and Biological Engineering,The Ohio State University, ⁵Department of Chemistry and Biochemistry,The Ohio State University, ⁶Department of Veterinary Preventive Medicine,The Ohio State University

Please note that some of the translations on this page are AI generated. Click here for the English version.

Overview

This study develops a methodology for effective drug delivery into the retina and optic nerves of adult rats, addressing a gap in standardized retrobulbar injection techniques. The research also innovates a tissue retrieval method for en bloc collection of the optic nerve and retina, minimizing postmortem injuries.

Key Study Components

Area of Science

Neuroscience
Ocular Pathologies
Drug Delivery Techniques

Background

Lack of standardized methodologies for retrobulbar injections in adult rats.
Importance of safe and effective delivery of therapeutics to posterior ocular tissues.
Need for improved tissue retrieval techniques that preserve optic nerve and retinal integrity.

Purpose of Study

To establish a reliable protocol for retrobulbar injections in adult rats.
To develop a top-down en bloc dissection technique for visualizing and retrieving optic tracks.
To expand literature on drug delivery methods and facilitate future therapeutic interventions.

Methods Used

The study utilized surgical platforms and live operating microscopes for precise injections.
Adult rats served as the biological model to perform retrobulbar injections and tissue retrieval.
No multiomics workflow was mentioned in the transcript.
Key steps included the use of specific needle gauges and injection techniques to minimize tissue damage.
Fluorescence imaging was used to assess the safety and effectiveness of the methods.

Main Results

Injection techniques ensured the delivery of therapeutics with minimal disruption to visual pathways.
Fluorescence staining confirmed the structural integrity of optic nerve tissues post-injection.
Some therapeutics were detected, informing future applications for ocular treatment.

Conclusions

The methodologies presented enhance the reliability of therapeutic interventions targeting ocular pathologies.
These techniques enable further investigation into neuroprotective drugs and their application in small animal models.
A clear understanding of injection safety and tissue retrieval improves assessments of neuronal mechanisms.

Frequently Asked Questions

What is the primary advantage of this study's methodology?

The study provides a standardized technique for retrobulbar injections, enhancing the reliability of drug delivery in experimental models.

How is the biological model implemented in this study?

Adult rats are used as the biological model, providing an effective platform for the technique of retrobulbar injections.

What types of outcomes can be obtained from the methodology?

This methodology allows for the assessment of drug delivery effectiveness and visual function integrity through FVEP testing.

How can this method be adapted for future research?

The techniques can be adjusted to explore various therapeutics for treating ocular injuries in small animal models.

What are key limitations of the study?

The study may face limitations in scalability to other species or models, as well as variability in therapeutic responses.

How does this research contribute to understanding ocular treatments?

By establishing safe and effective protocols, it enables a clearer exploration of therapeutic interventions for ocular pathologies.

Este estudo apresenta uma metodologia para a administração de terapêutica na retina e nos nervos ópticos do rato adulto. Além disso, um método exclusivo de recuperação de tecido é introduzido para uma coleção em bloco de cima para baixo do nervo óptico e da retina em um rato adulto.

O escopo da pesquisa é desenvolver técnicas de injeção para administrar medicamentos na parte posterior do olho para tratar várias patologias oculares. Também queríamos desenvolver uma técnica para visualizar toda a trilha óptica em bloco.

A literatura atual carece de uma metodologia padronizada para a injeção retrobulbar em ratos adultos. Este protocolo é estabelecer essa metodologia e, ao mesmo tempo, fornecer um procedimento de cima para baixo para obter amostras de tecido em bloco de toda a trilha neurovisual.

View the full transcript and gain access to thousands of scientific videos